Matrixome iMatrix 511-SILK Stem Cell Culture Substrate

Code: NP892-02
  • $518.00
  • (Delivery from $70.00)
Available | Usually dispatched within 24 hours
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Recombinant Laminin-511 E8-Fragments, expressed in Silkworm

iMatrix-511 SILK is a highly purified and refined laminin-511 E8 fragments, produced in Silkworms.

iMatrix-511 SILK features make it an ideal matrix for pluripotent stem cell culture:

  • Promotes greater stem cell adhesion than all other matrix proteins that have been tested
  • Easy to use (liquid format)
  • E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511
  • Equivalent performance, but lower cost than the legacy iMatrix-511 product

Laminin is localized to the basement membrane and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells.

 iMatrix-511 SILK  is functionally equivalent to iMatrix-511 (Cat. No. NP892-011). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511 is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes.

Product Name

iMatrix-511 SILK Stem Cell Culture Substrate

Catalog Number

NP892-021

Size

  • NP891-021: 6 × 175 µg

Molecular Weight

150 kDa

Purity

>95% pure

Formulation

Purified Laminin-511 E8 proteolytic fragment

Storage and Stability

Store at 4 °C and protect from light exposure. Stable for 2 years from the manufacturing date.

Concentration

500 µg/mL

Source

Silkworms

Quality Control

Integrin binding Kd < 10 nM

Sterility

Sterile

Notice To Purchaser

REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage

iMatrix-511-SILK is suitable for use as a substrate for the culture of various cell types, including ES/iPS cells.

Manufacturer

Matrixome Corporation (Japan)

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun.474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1". Stem Cell Reports6  (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells". Differentiation2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development from human iPS cells and recovery of corneal function". Nature531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ cell fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci.56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells". Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells." Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins." J. Biol. Chem.284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins." J. Biol. Chem.282 (15): 11144-54 (2017).

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