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Recombinant human PDGF-BB protein

QK044

Brand: Qkine

Human PDGF-BB (platelet derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. Its is a potent mitogen expressed by platelets which stimulates proliferation and angiogenesis and is involved in tissue repair. PDGF-BB increases stem cell proliferation and marker expression and can be used in osteogenic and neural stem cell differentiation.

Qkine recombinant human PDGF-BB is a highly pure 25 kDa disulfide–linked bioactive dimeric protein, animal origin-free (AOF), tag free and carrier protein-free.

Qkine 3-for-2 product campaign

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
Recombinant human PDGF-BB protein, 25 µg QK044-0025 25 µg (select above) $ 280.00 £ 205.00 € 240.00
Recombinant human PDGF-BB protein, 50 µg QK044-0050 50 µg (select above) $ 410.00 £ 305.00 € 357.00
Recombinant human PDGF-BB protein, 100 µg QK044-0100 100 µg (select above) $ 620.00 £ 455.00 € 532.00
Recombinant human PDGF-BB protein, 500 µg QK044-0500 500 µg (select above) $ 2,500.00 £ 1,840.00 € 2,150.00
Recombinant human PDGF-BB protein, 1000 µg QK044-1000 1000 µg (select above) $ 3,950.00 £ 2,900.00 € 3,388.00

Note: prices shown do not include shipping and handling charges.

Qkine company name and logo are the property of Qkine Ltd. UK.

Alternative protein names
GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor
Species reactivity

human

species similarity:
mouse – 89%
rat – 88%
porcine – 95%
bovine – 91%

Alternative:

Recombinant human PDGF-AA protein (Qk043)

Summary

  • High purity human PDGF-BB protein (Uniprot: P01127)
  • >98%, by SDS-PAGE quantitative densitometry
  • 25 kDa
  • Expressed in E. coli.
  • Animal origin-free (AOF) and carrier protein-free.
  • Manufactured in our Cambridge, UK laboratories
  • Lyophilized from acetonitrile, TFA
  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Handling and Storage FAQ

Featured applications

  • Neuronal differentiation
  • Osteogenic differentiation
  • Stem cell proliferation

Bioactivity

Human LIF Qk036 protein bioactivity lot #14293

PDGF-BB activity was determined by the proliferation of Balb/c 3T3 cells. Cells were treated in triplicate with a serial dilution of PDGF-BB for 3 days, PDGF-BB activity was determined using the CellTiter-Glo (Promega) luminescence assay. EC50 = 14.9 pM (0.37 ng/mL). Data from Qk044 lot #104306.

Purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

PDGF-BB migrates as a single band at ~30 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk044 batch #104306.

Further quality assays

  • Mass spectrometry: single species with expected mass
  • Analytical reversed-phase: single sharp peak
  • Endotoxin: <0.005 EU/μg protein (below level of detection)
  • Recovery from stock vial >95%

Qkine PDGF-BB is more biologically active than a comparable alternative supplier protein

Quantitative luciferase assay with Qkine PDGF-BB (Qk044, green) and alternative supplier PDGF-BB (Supplier B, black). Cells were treated in triplicate with a serial dilution of PDGF-BB for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.

Protein background

Platelet derived growth factor (PDGF) is part of the PDGF family. As the name suggests it is produced by platelets, and by several other cell types including endothelial cells, fibroblasts, vascular smooth muscle cells, osteoblasts, glia and neurons [1]. In platelets, PDGF is stored in α-granules, and released in response to platelet activation, leading to stimulation of epithelial cells. PDGF is a potent mitogen for cells of mesenchymal origin such as fibroblasts, glial cells and vascular smooth muscle cells.

PDGFs are dimers of PDGF-A, -B, -C or -D polypeptide chains, arranged in homodimers (e.g. PDGF-AA and PDGF-BB) or heterodimers (e.g. PDGF-AB). There are two receptors for PDGFs, PDGFR-α and PDGFR-β. PDGFR-α is a receptor for all PDGF dimers apart from PDGF-DD, while PDGFR-β is a receptor for PDGF-BB and PDGF-DD [2]. Activation of these receptors by PDGF leads to activation of downstream signalling pathways including PI3K, MAPK and STAT3 pathways [3]. PDGF plays an important role in embryonic development [4], cell proliferation, cell migration and angiogenesis. PDGF is a potent mitogen for cells of mesenchymal origin such as fibroblasts, glial cells and vascular smooth muscle cells.

PDGF-BB promotes stem cell proliferation and stem cell marker expression, it can promote migration of stem cells, such as mesenchymal stem cells (MSCs) and increases cell viability in stem cell spheroids from human bone marrow-derived stem cells [5].  PDGF-BB acts as a neurotrophic factor and is involved in the proliferation and differentiation of neuronal progenitor cells [6]. It can be used in the neuronal differentiation of urine-derived stem cells (USCs) where it stimulates the trans-differentiation of USC into neuronal cells [6]. Clinically, PDGF-BB can be used in the treatment of periodontal and orthopedic bone defects and dermal wound healing [7].

Background references

  1. Demoulin, Jean-Baptiste, and Carmen P. Montano-Almendras. ‘Platelet-Derived Growth Factors and Their Receptors in Normal and Malignant Hematopoiesis’. American Journal of Blood Research, vol. 2, no. 1, Jan. 2012, pp. 44–56. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3301440/
  2. Kardas, Grzegorz, et al. ‘Role of Platelet-Derived Growth Factor (PDGF) in Asthma as an Immunoregulatory Factor Mediating Airway Remodeling and Possible Pharmacological Target’. Frontiers in Pharmacology, vol. 0, 2020. doi:10.3389/fphar.2020.00047.
  3. Demoulin, Jean-Baptiste, and Ahmed Essaghir. ‘PDGF Receptor Signaling Networks in Normal and Cancer Cells’. Cytokine & Growth Factor Reviews, vol. 25, no. 3, June 2014, pp. 273–83. doi:10.1016/j.cytogfr.2014.03.003.
  4. Andrae, Johanna, et al. ‘Role of Platelet-Derived Growth Factors in Physiology and Medicine’. Genes & Development, vol. 22, no. 10, May 2008, pp. 1276–312. doi:10.1101/gad.1653708.
  5. Paek SC, Min SK, Park JB. Effects of platelet-derived growth factor-BB on cellular morphology and cellular viability of stem cell spheroids composed of bone-marrow-derived stem cells. Biomed Rep. 2020 Dec;13(6):59. doi: 10.3892/br.2020.1366
  6. Kim, Jung Yeon, et al. ‘Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells’. Tissue Engineering and Regenerative Medicine, vol. 15, no. 2, Dec. 2017, pp. 195–209. doi:10.1007/s13770-017-0102-x.
  7. Tavelli, L., et al. ‘Recombinant Human Platelet–Derived Growth Factor: A Systematic Review of Clinical Findings in Oral Regenerative Procedures’. JDR Clinical & Translational Research, vol. 6, no. 2, Apr. 2021, pp. 161–73. doi:10.1177/2380084420921353.