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Recombinant human KGF (FGF-7) protein

QK046

Brand: Qkine

Human KGF protein (FGF-7)  is a member of the FGF family. KGF protein is used in the culture of lung, breast, liver, and bladder organoids.

Qkine human FGF-7 / KGF is a highly pure and bioactive 19 kDa protein, animal-origin free (AOF) and carrier-protein free (CF).

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
Recombinant human KGF (FGF-7) protein, 25 µg QK046-0025 25 µg (select above) $ 355.00 £ 255.00 € 298.00
Recombinant human KGF (FGF-7) protein, 50 µg QK046-0050 50 µg (select above) $ 515.00 £ 380.00 € 444.00
Recombinant human KGF (FGF-7) protein, 100 µg QK046-0100 100 µg (select above) $ 760.00 £ 560.00 € 655.00
Recombinant human KGF (FGF-7) protein, 500 µg QK046-0500 500 µg (select above) $ 3,100.00 £ 2,300.00 € 2,687.00
Recombinant human KGF (FGF-7) protein, 1000 µg QK046-1000 1000 µg (select above) $ 4,950.00 £ 3,600.00 € 4,205.00

Note: prices shown do not include shipping and handling charges.

Qkine company name and logo are the property of Qkine Ltd. UK.

Alternative protein names
Fibroblast growth factor 7, Keratinocyte growth factor, KGF, Keratinocyte growth factor 1, KGF1 HBGF-7, Heparin-binding growth factor 7
Species reactivity
Mature human FGF-7, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF

Frequently used together:

Summary

  • High purity KGF (FGF-7) protein (Uniprot: P21781)
  • 19 kDa>98%, by SDS-PAGE quantitative densitometry
  • Expressed in E. coli
  • Animal origin-free (AOF) and carrier protein-free
  • Manufactured in Qkine Cambridge, UK laboratories
  • Lyophilized from PBS
  • Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Handling and Storage FAQ

Featured applications


  • Airway, mammary, liver hepatocyte, and urothelium organoids

Bioactivity

FGF-7 (KGF) activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 92 pM (1.75 ng/mL). Data from Qk046 lot #104314. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Qk046-Datasheet-graph_-FGF7

Purity

KGF protein migrates as a monomer at 19 kDa and a dimer at 38 kDa  in non-reducing (NR) conditions and 19 kDa upon reduction (R).  No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk046 lot #104314

210623-SDS-PAGE-FGF7-104314-3-ug

Further quality assays

  • Mass spectrometry: single species with expected mass
  • Endotoxin: <0.005 EU/μg protein (below level of detection)
  • Recovery from stock vial:  >95%

Comparison with other suppliers

Qk046-FGF7-800

 

KGF (FGF-7) from Qkine (Qk046) and a major supplier have equivalent bioactivity. Qk046 KGF (FGF-7) lot #104425 and KGF from another supplier were compared in the same assay plate with EC50s of 2.0 ng/ml and 3.7 ng/ml respectively.

Protein background

Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and, alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. This subfamily is secreted exclusively by the mesenchyme, and specifically activates the “b” isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) present in the overlying epithelium [1], of these receptors KGF acts through the IIIb splice form of FGFR2. KGF is required for the development of the kidney, thymus, and hippocampus [2]. KGF also plays an import role in wound healing [3], a role exemplified in studies on bioengineered skin [3]. KGF protein has been used in generation of many organoids including lung, breast, liver and bladder [2].

Background references

  1. Zinkle, Allen, and Mohammadi, Moosa. ‘Structural Biology of the FGF7 Subfamily’. Frontiers in Genetics, vol. 10, 2019. Frontiers, doi:10.3389/fgene.2019.00102.
  2. Fujii, Masayuki, and Sato, Toshiro. ‘Somatic Cell-Derived Organoids as Prototypes of Human Epithelial Tissues and Diseases’. Nature Materials, vol. 20, no. 2, Feb. 2021, pp. 156–69. doi:10.1038/s41563-020-0754-0.
  3. Werner, Sabine, and Grose, Richard. ‘Regulation of Wound Healing by Growth Factors and Cytokines’. Physiological Reviews, vol. 83, no. 3, July 2003, pp. 835–70. doi:10.1152/physrev.2003.83.3.835
  4. Erdag, Gulsun, et al. ‘FGF-7 Expression Enhances the Performance of Bioengineered Skin’. Molecular Therapy, vol. 10, no. 1, July 2004, pp. 76–85. doi:10.1016/j.ymthe.2004.04.013.

Publications using recombinant human KGF (FGF-7) protein (Qk046)

Generation of human iPSC-derived pancreatic organoids to study pancreas development and disease
Darrigrand J-F, Isaacson A and Spagnoli FM
DOI: https://doi.org/10.1101/2024.10.24.620102