Improving human health through Biomedical innovation and discovery.

Recombinant human/ mouse/ rat/ bovine/ porcine BMP-2 protein

QK007

Brand: Qkine

Human recombinant BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.

26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein, animal origin-free (AOF) and carrier protein-free.

Currency: 

Product name Catalog number Pack size Price Price (USD) Price (GBP) Price (EUR)
Recombinant human/mouse/rat/bovine/porcine BMP-2 protein, 25 µg QK007-0025 25 µg (select above) $ 280.00 £ 205.00 € 240.00
Recombinant human/mouse/rat/bovine/porcine BMP-2 protein, 50 µg QK007-0050 50 µg (select above) $ 410.00 £ 305.00 € 357.00
Recombinant human/mouse/rat/bovine/porcine BMP-2 protein, 100 µg QK007-0100 100 µg (select above) $ 620.00 £ 455.00 € 532.00
Recombinant human/mouse/rat/bovine/porcine BMP-2 protein, 500 µg QK007-0500 500 µg (select above) $ 2,500.00 £ 1,840.00 € 2,150.00
Recombinant human/mouse/rat/bovine/porcine BMP-2 protein, 1000 µg QK007-1000 1000 µg (select above) $ 3,950.00 £ 2,900.00 € 3,388.00

Note: prices shown do not include shipping and handling charges.

Qkine company name and logo are the property of Qkine Ltd. UK.

Alternative protein names
Bone morphogenetic protein 2,BDA2, SSFSC, BMP-2A, BMP-2, Bone morphogenetic protein 2A
Species reactivity

human

species similarity:
mouse – 100%
rat – 100%
porcine – 100%
bovine – 100%

Summary

  • High purity human bone morphogenetic protein 2, BMP-2 (Uniprot: P12643)
  • 26 kDa (dimer)
  • >98%, by SDS-PAGE quantitative densitometry
  • Expressed in E. coli
  • Animal origin-free (AOF) and carrier protein-free
  • Manufactured in Qkine's Cambridge, UK laboratories
  • Lyophilized from acetonitrile, TFA
  • Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Handling and Storage FAQ

Featured applications

  • Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes

Bioactivity

BMP-2 activity is determined using the BMP-2 responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of BMP-2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.3 nM (7.8 ng/mL). Data from Qk007 lot #10.

Qk007-bioactivity

 

Purity

BMP-2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk007 lot #010

human-BMP2-Qk007-protein-purity-lot-010

Further quality assays

  • Mass spectrometry: single species with expected mass
  • Analytical reversed-phase: single sharp peak
  • Endotoxin: <0.005 EU/μg protein (below level of detection)
  • Recovery from stock vial:  >95%

20241015-BMP2-bioassay-comparison-800

Qkine BMP-2 is more biologically active than a comparable alternative supplier protein. Bioactivity was determined using a BMP-2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of Qkine BMP-2 (Qk007, green) or alternative BMP-2 (supplier B, black). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk007 lot #204510.

Protein background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP-2 protein is a potent differentiation factor and directs human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes [1] .  Recombinant BMP-2 protein induces bone and cartilage formation in vitro and chondrogenesis in human adult mesenchymal stem cells [2].

Human bone morphogenetic protein 2 (BMP-2) is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP-2 protein to form active disulfide-linked homodimers, and heterodimers with BMP7.

Mature human BMP-2 protein shares 100% amino acid sequence identity with mouse and rat BMP-2. It also shares 85% amino acid sequence identity with the related protein, BMP-4, and less than 51% identity with other BMPs.

Background references

  1. Pera, M. F. et al. Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin. J. Cell Sci. 117, 1269–80 (2004). doi.org/10.1242/jcs.00970
  2. Schmitt, B. et al. BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture. Differentiation. 71, 567–77 (2003). doi.org/10.1111/j.1432-0436.2003.07109003.x

Publications using Recombinant human BMP-2 protein (Qk007)

CD1530, selective RARγ agonist, facilitates Achilles tendon healing by modulating the healing environment including less chondrification in a mouse model
Yimiti D, Uchibe K, Toriyama M et al.
DOI: 10.1002/jor.26006

High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans
Stucchi S, Sepulveda-Rincon LP, Dion C et al.
DOI: https://doi.org/10.1101/2025.01.14.632914

Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells
Luo L, Foster NC, Man KL et al.
DOI: 10.1002/biot.202100401

Sex-specific chromatin remodelling safeguards transcription in germ cells
Huang TC, Wang YF, Vazquez-Ferrer E et al.
DOI: 10.1038/s41586-021-04208-5