Purchase product: USA Store
|Product Code||Pack Size||Price in
|Price in EMEA|
|03-0007||10 μg||USD $ 219.00||GBP £ 169.55||EUR € 192.44|
Prices do not include shipping and handling charges.
Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGFβ protein super-family. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis2,3. BMP-4 plays an important role in the differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human pluripotent stem cells5. Stemfactor BMP-4 is a recombinant protein expressed and purified from human 293 cells as a glycosylated homodimer with a molecular mass of 34 kDa.
Product Name: Stemfactor BMP-4, Human Recombinant
Catalog Number: 03-0007
Size: 10 µg
Purity: Greater than 95% by SDS-PAGE analysis.
Formulation: Lyophilized from sterile-filtered 1 M NaCl, 50 mM NaOAc, pH 4.5.
Storage and Stability: Stemfactor BMP-4 is shipped at room temperature. Lyophilized BMP-4 is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C.
Sterility: Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.
Source: Stemfactor BMP-4 was expressed in and purified from human 293 cells.
Amino Acid Sequence: SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
Uniprot Accession Number: P12644, residues 293-408
Endotoxin Level: Less than 1.0 EU/µg of BMP-4 as determined by the LAL method.
Biologic Activity: The ED50 is less than 30 ng/mL as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells.
Intended use: This product is intended for research use only, and is not for therapeutic or diagnostic use in humans.
Stem Cell Reagents
3D Cell Culture
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