Stemfactor™ Activin A, Human Recombinant

03-0001

Brand: Stemgent™

Purchase product: USA Store

Product Code Pack Size Price in
The Americas
Price in EMEA
03-0001 5 μg USD $ 158.00 GBP £ 122.01 EUR € 138.49

Prices do not include shipping and handling charges.

stemfactor-activin-a-human-recombinant-p202-161_mediumStemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naïve pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor LIF, Human Recombinant (03-0016), and

A: Stemfactor Activin A, Human Recombinant (03-0001)

Specifications

Product Name: Stemfactor Activin A, Human Recombinant

Catalog Number: 03-0001

Size: 5 µg

Purity: Greater than 98% by SDS-PAGE analysis

Formulation: Lyophilized from 20% acetonitrile

Reconstitution: Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.

Storage and Stability: Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.

References

  1. Beattie, G.M., Lopez, A.D., Bucay, N., Hinton, A., Firpo, M.T., King, C.C., and Hayek, A. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23: 489-495 (2005).
  2. Vallier, L., Alexander, M., and Pedersen, R.A. Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509(2005).
  3. Sulzbacher, S., Schroeder, I.S., Truong, T.T., and Wobus, A.M. Activin A-induced differentiation of embryonic stem cells into endoderm and pancreatic progenitors- the influence of differentiation factors and culture conditions. Stem Cell Rev 5: 159-173(2009). 
  4. Valdimarsdottir, G., and Mummery, C. Functions of the TGFβ superfamily in human embryonic cells. APMIS 113: 773-789(2005).

Additional Publications