Stemfactor Activin A is a crystallography-grade preparation providing the highest purity available. Activin A is bi-modal in action having been shown to maintain pluripotency of stem cells and promote differentiation1,2,3,4. Mature recombinant human Activin A is a ~25 kDa disulfide-linked homodimer of two 116 amino acid residue βA subunits.
Specifications
Product Name: Stemfactor Activin A, Human Recombinant
Catalog Number: 03-0001
Size: 5 µg
Purity: Greater than 98% by SDS-PAGE analysis
Formulation: Lyophilized from 20% acetonitrile
Reconstitution: Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability: Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted Activin A, at concentrations greater than 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and for 1 year when stored at −80 °C.
Documents
Specification Sheets:
Safety Data Sheets:
References
- Beattie, G.M., Lopez, A.D., Bucay, N., Hinton, A., Firpo, M.T., King, C.C., and Hayek, A. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 23: 489-495 (2005).
- Vallier, L., Alexander, M., and Pedersen, R.A. Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J Cell Sci 118: 4495-4509(2005).
- Sulzbacher, S., Schroeder, I.S., Truong, T.T., and Wobus, A.M. Activin A-induced differentiation of embryonic stem cells into endoderm and pancreatic progenitors- the influence of differentiation factors and culture conditions. Stem Cell Rev 5: 159-173(2009).
- Valdimarsdottir, G., and Mummery, C. Functions of the TGFβ superfamily in human embryonic cells. APMIS 113: 773-789(2005).
Additional Publications
- Adkar SS; Wu C-L; WIllard VP; Dicks A; Ettyreddy A; Steward N; Bhutani N; Gersbach CA; Guilak F. Step‐Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR‐Cas9 Genome Editing. Stem Cells 37:65-76 (2019).
- Mobarra N; Soleimani M Ghayour-Mobarhan M; Safarpour S; Ferns GA; Pakzad R; Pasalar P. Hybrid poly‐l‐lactic acid/poly(ε‐caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell‐derived hepatocyte‐like cells. J Cell Physiol :doi.org/10.1002/jcp.27779 (2018).
- Rajam KAN; Khater M; Soncin F; Pizzon D; Moretton-Zita M; Pham J; Stus O; Iyer P; Tache V; Laurent LC; Parast MM. Sirtuin1 is required for proper trophoblast differentiation andplacental development in mice. Placenta 62:43108 (2018).
- Yucer N; Holzapfel M; Jenkins Vogel T; Lenaeus L; Ornelas L; Laury A; Sareen D; Barrett R; Karlan BY; Svendsen CN. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium. Sci Rep 7:10741 (2017).
- Villa-Diaz LG; Kim JK; Laperle A; Palecek SP; Kresbach PH. Inhibition of focal adhesion kinase signaling by integrin a6b1 supports human stem cell self-renewal. Stem Cells doi: 10.1002/stem.2349 (2016).
- Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. Human pancreatic β-like cells converted from fibroblasts. Nature Commun 7:10080 (2016).
- Saxena S; Ronn RE; Guibentif C; Moragheba R; Woods N-B. Cyclic AMP signaling through Epac axis modulates human hemogenic endothelium and enhances hematopoeitic cell generation. Stem Cell Rep doi:10.1016/j.stemcr.2016.03.006 (2016).
- Mora-Castilla S; To C; Vaezeslami S; Morey R; Srinivasan S; Dumdie JN; Cook-Andersen H; Jenkins J; Laurent LC. Miniaturization technologies for efficient single-cell library preparation for next-generation sequencing. J Lab Automation 2016:1 (2016).
- Qian X; Kim JK; Tong W; Villa-Diaz LG; Krebsbach PH. DPPA5 supports pluripotency and reprogramming by regulating NANOG turnover. Stem Cells 34:588-600 (2016).
- Qian X. "Defining the mechanism by which synthetic polymer surfaces support human pluripotent stem cell self-renewal." PhD Thesis, University of Michigan (2015).
- Villa-Diaz LG; Kim JK; Lahann J; Kresbach PH. Derivation and long-term culture of transfene-free human induced pluripotent stem cells on synthetic substates. Stem Cells Trans Med 3:1410 (2014).
- Bukong, T.N., Lo, T., Szabo, G., and Dolganiuc, A. Novel developmental biology-based protocol of embryonic stem cell differentiation to morphologically sound and functional yet immature hepatocytes. Liver Int doi: 10.1111/j.1478-3231.2011.02743.x. [Epub ahead of print] (2012).
- Kunisada, Y., Tsubooka-Yamazoe, N., Shoji, M., and Hosoya, M. Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells. Stem Cell Res 8: 274-284 (2012).
- McCracken, K.W., Howell, J.C., Wells, J.M., and Spence, J.R. Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6: 1920-1928 (2011).