iMatrix-332 Corneal Epithelial Cell Culture Substrate

iMatrix-332's features make it an ideal matrix for some cell culture applications:

• Xeno-free formulation / CHO-S cell bioproduction
• Easy to use (liquid format)
• E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-221

iMatrix-332 is a highly purified and refined product of human recombinant laminin-332 (E8 fragment) expressed by CHO-S cells. Laminin 332 supports cells in the epithelial basement membranes, lining the surfaces of the body such as the skin, hair follicles, oral cavity, gastrointestinal and urinary tracts, lungs, and different glands. The association of cells with laminin 332 occurs through α3β1 integrin in focal adhesions and α6β4 in stable anchoring contacts, which contain an assembly of hemidesmosome proteins.

Laminin-332 is an important component of the "skin basement membrane" that exists between the epithelium and dermis of the skin, and is also expressed in human retinal pigment epithelium (RPE) and hair follicle cells.

Matrixome company name and logo and iMatrix brand name are the property of Matrixome Corp., Japan.

Product Name: iMatrix-332 Corneal Epiethelial Cell Culture Substrate

Catalog Numbers:

• NP892-031
• NP892-032

Sizes:

• NP892-031 — 2 × 175 µg
• NP892-032 — 6 × 175 µg

Molecular Weight: 150 kDa

Purity: > 95 % pure

Formulation: Purified Laminin-332 E8 proteolytic fragment

Storage and Stability: Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.

Quality Control: Activity: K_d for integrin binding

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage: iMatrix-332 is suitable for use as a substrate for culture of various cell types.

Concentration: 500 µg/mL

Source: CHO-S cell expression

Manufacturer: Matrixome Corporation (Japan)

Safety Data Sheets:

• NP892-03 Manual
• NP892-03 Safety Data Sheet

Product Flyer:

• iMatrix™ Cell Culture Substrates

Additional Publications

• Sekine H; Homma J; Shimizu T. Tricultured cell sheets develop into functional pancreatic islet tissue with a vascular network. Research Square doi.org/10.21203/rs.3.rs-1900386/v1: (2022).