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iMatrix-511 SILK Stem Cell Culture Substrate

NP892-021

Brand: iMatrix™ by Matrixome®

An alternative iMatrix-511 that is xeno-free, recombinant Laminin-511 E8 Fragment expressed in silkworm for promoting adherence and culture of human iPS cells.


Note: prices shown do not include shipping and handling charges.

Product code Pack size The Americas   UK, Europe, Middle East, Africa
(USD)   UK (GBP) Europe (EUR)

NP892-021

175 μg × 6 tubes

$ 578.00

 

£ 518.70

€ 588.74

  USA store  
The Americas
Product code Pack size USD

NP892-021

175 μg × 6 tubes

$ 578.00

USA store
UK, Europe, Middle East, Africa
Product code Pack size UK (GBP)

NP892-021

175 μg × 6 tubes

£ 518.70

Europe (EUR)

NP892-021

175 μg × 6 tubes

€ 588.74


imatrix-511-silk-stem-cell-culture-substrate-p471-392_medium

An alternative iMatrix-511 that is xeno-free, recombinant Laminin-511 E8 Fragment expressed in silkworm for promoting adherence and culture of human iPS cells. iMatrix-511 SILK is similar in performance, but lower in cost relative to NP892-011.

iMatrix-511 SILK is a highly purified and refined laminin-511 E8 fragments, produced in Silkworms.

iMatrix-511 SILK features make it an ideal matrix for pluripotent stem cell culture:

  • Promotes greater stem cell adhesion than all other matrix proteins that have been tested
  • Easy to use (liquid format)
  • E8 fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511
  • Equivalent performance, but lower cost than the legacy iMatrix-511 product

Laminin is localized to the basement membrane and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types including stem and iPS cells.

 iMatrix-511 SILK  is functionally equivalent to iMatrix-511 (Cat. No. NP892-011). The only difference is the expression system used for bioproduction. The iMatrix-511 SILK product is produced in recombinant silkworm cocoon, while iMatrix-511 is produced in CHO-S cells. Both are E8 fragments that have been purified using the same processes.

Specifications

Product Name: iMatrix-511 SILK Stem Cell Culture Substrate

Catalog Number: NP892-021

Size: 6 × 175 µg

Molecular Weight: 150 kDa

Purity: > 95 % pure

Formulation: Purified Laminin-511 E8 proteolytic fragment

Storage and Stability: Store at 4 °C and protect from light exposure. Stable for 2 years from the manufacturing date.

Concentration: 500 µg/mL

Source: Silkworms

Quality Control: Integrin binding Kd < 10 nM

Sterility: Sterile

Notice To Purchaser: REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market.

Recommended Usage: iMatrix-511-SILK is suitable for use as a substrate for the culture of various cell types, including ES/iPS cells.

Manufacturer: Matrixome Corporation (Japan)

Documents

Safety Data Sheet:

Specifications Sheets:

Product Flyer:

References

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun.474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1". Stem Cell Reports6  (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells". Differentiation2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development from human iPS cells and recovery of corneal function". Nature531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ cell fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci.56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells". Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells." Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins." J. Biol. Chem.284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins." J. Biol. Chem.282 (15): 11144-54 (2017).

Additional Publications