Sox2 mRNA, Human

05-0016

Brand: Stemgent™

Purchase product: USA Store

Product Code Pack Size Price in
The Americas
Price in EMEA
05-0016 20 μg USD $ 250.00 GBP £ 193.55 EUR € 219.68

Prices do not include shipping and handling charges.

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Stemgent Sox2 mRNA encodes the Sox2 protein commonly used in cellular reprogramming systems for the generation of induced pluripotent stem (iPS) cells. This in vitro transcribed mRNA incorporates both pseudouridine and 5-methylcytidine modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2Repeated transfection of Sox2 mRNA in combination with mRNAs encoding Oct4, Klf4, Lin-28 and c-Myc has been shown to reprogram human fibroblasts.2

1536842563050018_fig1

Figure 1: Protein expression and localization shown by immunocytochemistry, DAPI in blue and Sox2 in red.

Specifications

Product Name: Sox2 mRNA, Human

Catalog Number: 05-0016

Formulation: 100 ng/µl in RNase-free TE buffer, pH 7.0

Storage and Stability: Store at or below −70 °C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (<5). Aliquot to experimental quantities.

Quality Control: Proper protein expression and localization have been confirmed by immunocytochemistry.

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Notice To Purchaser: This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic and prognostic use, (iii) any use in delivery to and/or modification of cells that are intended for clinical, diagnostic or medicinal use, including without limitation, cell-based therapy, (iv) any commercial purposes, including without limitation the performance of contract research or provision of services to a third party and the manufacture of products for general sale. Any use of this product for any of the abovementioned purposes requires a license from the Massachusetts Institute of Technology.

Recommended Usage: Stemgent Sox2 mRNA, Human should be transfected using a protocol specifically optimized for the cell type used in the experiment.

Documents

Manuals

Product Sheets

References

  1. Angel, M., and Yanik, M.F. (2010) Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS One 5: e11756.