Recombinant human GDF-15 protein
QK017
Brand: Qkine
Human growth differentiation factor 15 (GDF-15) protein is a member of the TGFβ family and subject of intense interest as a marker of cellular stress and for its role in metabolism, cancer and pregnancy. Human GDF-15 also is functional in mouse studies.
Qkine GDF-15 is a 25 kDa disulfide-linked dimer composed of the mature domain of human GDF-15 protein. Our recombinant GDF-15 protein is exceptionally high purity, animal origin-free and extensively validated to ensure no trace contamination of related TGF-β family proteins from the mammalian culture systems.
Currency:
| Product name | Catalog number | Pack size | Price | Price (USD) | Price (GBP) | Price (EUR) |
|---|---|---|---|---|---|---|
| Recombinant human GDF-15 protein, 25 µg | QK017-0025 | 25 µg | (select above) | $ 280.00 | £ 205.00 | € 240.00 |
| Recombinant human GDF-15 protein, 50 µg | QK017-0050 | 50 µg | (select above) | $ 410.00 | £ 305.00 | € 357.00 |
| Recombinant human GDF-15 protein, 100 µg | QK017-0100 | 100 µg | (select above) | $ 620.00 | £ 455.00 | € 532.00 |
| Recombinant human GDF-15 protein, 500 µg | QK017-0500 | 500 µg | (select above) | $ 2,500.00 | £ 1,840.00 | € 2,150.00 |
| Recombinant human GDF-15 protein, 1000 µg | QK017-1000 | 1000 µg | (select above) | $ 3,950.00 | £ 2,900.00 | € 3,388.00 |
Note: prices shown do not include shipping and handling charges.
Qkine company name and logo are the property of Qkine Ltd. UK.
Alternative protein names
Species reactivity
human
species similarity:
mouse – 67%
porcine – 67%
rat – 66%
bovine – 66%
Bioactivity
GDF-15 signals through GRAL and co-receptor RET leading to RET phosphorylation and signalling through the ERK and AKT pathway (reviewed in Emmerson et al., 2018). Commercial sources of GDF-15, in particular those purified from mammalian expression systems, have been shown previously to be contaminated with trace amounts of TGF-β. These trace contaminants cause misleading experimental results due to the picomolar or even femtomolar EC50s (Olsen et al., 2017). Here we use a well-characterized SMAD2/3 activation assay to show that there is no contamination from other TGF-β family proteins. Bioactivity is determined using a luciferase reporter assay in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of GDF-15 or Qk010 TGF-β1 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0 pM (no contamination with TGF-β or related growth factors). Data from Qk017 lot #104282
Purity
GDF-15 migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk017 lot #010