A "physically strong" collagen sponge (96-well plate size) derived from bovine dermal type I collagen that shows resistance to compressive loading. Culturing with dynamic stimulus (such as pressure) can mimic in-vivo-like cell response as shown in some arthritic cell culture models.
Applications
- Cell culture under dynamic stimuli
- 3D cell culture
- Carrier for cell transplant
Features
- Retains structural rigidity during repeated compression loading of 10-20 kPa
- Sized for 96 well plates
Data
"Mechanical stimulus culture assay using MIGHTY." (K. Shimomura, K. Nakata et al. Osaka University, Faculty of Medicine)
Forty kPa of repeated compressive load for 1 hour induced the expression of PEG2, IL6 and IL8, which are correlated to arthritis, after three days cultivation on MIGHTY.
Dexamethasone (Dex), anti-inflammatory agent, reduced the expression of PEG2, IL6 and IL8 in a concentration-dependent manner. These results indicate MIGHTY can be used for in vitro arthritis models.
Specifications
Product Name: Atelocollagen sponge, MIGHTY
Catalog Numbers:
- KKN-CSM-25
- KKN-CSM-50
Sizes:
- KKN-CSM-25 — 25 pieces
- KKN-CSM-50 — 50 pieces
Sponge Dimensions: 5 mm diameter × 3 mm sponges
Storage and Stability: Store at Room Temperature
Sterility: Sterile
Notice To Purchaser: REPROCELL is a licensed global distributor of KOKEN’s collagen-derived products everywhere, except for Japan.
Manufacturer: Koken Co., Ltd. (Japan)
References
- Shiozaki Y, et al. Enhanced in vivo osteogenesis by nanocarrier-fused bone morphogenetic protein-4. (2013) Int J Nanomedicine. 8:1349-1360.
- Akamine Y, et al. Prolonged matrix metalloproteinase-3 high expression after cycliccompressive load on human synovial cells in three-dimensional cultured tissue. (2012) Int J Oral Maxillofac Surg. 41(7):874-881.
- Muroi Y, et al. Effects of compressive loading on human synovium-derived cells. (2007) J Dent Res. 86(8):786-791.
