A nearly transparent permeable membrane made from Type 1 Atellocolagen sized for 24-well culture plates. Amino acids and other small molecules can move freely through the permeable collagen membrane. Suitable for culture of primary epithelial cells and cell interaction studies.
Applications
Features
- Microscopic observation of the cells is possible during the culture due to high membrane transparency.
- Cell culture on collagen membrane creates a natural environment that resembles in vivo systems.
Data
Induction into layered tissue from mouse embryoid body on permeable collagen membrane and transplantation. (Y. Kinebuchi et al. Shinonoi General Hospital, Shinshu University, Faculty of Medicine)
Mouse embryoid body was cultivated on permeable collagen membrane in a mixed medium of keratinocyte serum-free medium and conditioned medium from 3T3 cells. These cells formed four-layered structures in vitro after 28 days culture (Fig. 1). Upon transplanting these four-layered structure, the formation of luminal walls and the expression of some epithelial cell marker, UPIII (urothelium), CK8, and CK18 (bladder) were observed in vivo (Fig. 2).
KOKEN company name and logo and AteloCell brand are the property of KOKEN Corp. Ltd., Japan.
Product Name: Atelocollagen membrane for 6 well culture plate
Catalog Number: KKN-CM-24
Size: 24 pieces
Storage and Stability: Store at −20°C
Sterility: Sterile
Notice To Purchaser: REPROCELL is a licensed global distributor of KOKEN’s collagen-derived products everywhere, except for Japan.
Manufacturer: Koken Co., Ltd. (Japan)
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