Stemfactor™ Hepatocyte Growth Factor, Human Recombinant

Recombinant Human Hepatocyte Growth Factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal stem cells and targets and acts primarily upon epithelial and endothelial cells, but also acts on haemopoietic progenitor cells and T-cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.

Hepatocyte growth factor regulates cell growth, cell motility, and mophogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis and tissue regeneration.

Recombinant human HGF is an approximate 79 kDa active form of human HGF, which is a disulfide-linked heterodimer composed of α-chain (458 amino acids) and β-chain (234 amino acids) subunits. It lacks the 5 amino acid residues (F-L-P-S-S) in the 1st kringle domain; and is supplied as a glycoprotein produced by CHO cells.

Stemgent and the Stemfactor brand name are trademarks of REPROCELL Inc., Japan.

Product Name: Stemfactor Hepatocyte Growth Factor, Human Recombinant

Catalog Number: 03-0019

Size:

• 25 µg (1 µg/µL)
• 250 µg (1 µg/µL)

Purity: Greater than 99.5 % by SDS-PAGE analysis.

Formulation: Dissolved in 10 mM Citrate buffer, 0.075% Tween 80, 1 M NaCl, pH 6.0. Sterilized by filtration through a 0.22 µm filter.

Storage and Stability: Store at −70 °C.

Quality Control: Single chain form HGF <2.5 % by SDS-PAGE under reducing conditions, visualized by Coomassie Brilliant Blue staining.

Sterility: Sterile filtered

Notice To Purchaser: This HGF material was produced as GMP-compatible material in a pilot scale-up run for GMP manufacturing. Custom size pharmaceutical grade GMP-compliant material is available from REPROCELL (please inquire).

Concentration: 25 µL, 1 µg/µL (Cat. No. 03-0019) 250 µL, 1 µg/µL (Cat. No. 03-0019-250)

Source: Recombinant human HGF expressed in CHO cells

Endotoxin Level: Less than 0.125 EU/µg of protein as determined by the LAL method.

Biologic Activity: Biological activity was assessed based on the ability to induce proliferation of mink lung epithelial (Mv.1.Lu cells).

Manufacturer: Kringle Pharmaceutical (Japan)

Specification sheet:

• Stemfactor HGF Spec Sheet

Safety Data Sheet:

• SDS

1. B. Rafferty, P. Maile, P. Rigsby, R.E. Gaines Das, and C.J. Robinson (2001) International standards for hepatocyte growth factor / scatter factor: initial assessment of candidate materials and their ealuation by multicentre collaborative study. Journal of Immunological Methods: 258, pp 1-11.
2. T. Seki, I. Ihara, A. Sugimura, M. Shimonishi, T. Nishizawa, O. Asam, M. Hagiya, T. Nakamura and S. Shimizu (1990) Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte. Biochemical and Biophysical Research Communications, Vol. 172, No.1, pp 321-327.