Very low passage mouse embryonic fibroblast feeder cells. Hugely popular cell type for feeder-dependent culture of iPSC from a wide range of species.
MEF (Mouse Embryonic Fibroblasts) feeder cells are applicable for a wide range of ES/iPS cell culture. ReproCELL offers exceptionally consistent quality thanks to rigorous validation testing. See protocol (below) for more information.
Features
- Mouse Embryonic Fibroblast feeder cells derived from mouse embryo.
- A feeder cell line applicable for culture of mouse, primate, human ES/iPS cells.
- Validated high quality with every lot.
- Mitomycin C-treated cells ready for use.
Specifications
Product Name: Feeder Cells (MEF)
Catalog Number: RCHEFC003
Size: (3 × 10⁶ cells) × 5 vials
Storage and Stability: Liquid nitrogen. This product is very thermosensitive. Upon delivery please move the cell vials into liquid nitrogen to ensure optimal cell attachment levels.
References
- Kobayashi H; Hatakeyama H; Nishimura N; Yokota M; Suzuki S; Tomabechi Y; Shirouzu M; Osada H; Mimaki M; Goto Y-i; Yoshida M. Chemical reversal of abnormalities in cells carrying mitochondrial DNA mutations. Nature Chem BIol in press://doi.org/10.1038/s41589-020-00676-4 (2020).
- Kishigami, Ryota, et al.Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development. Journal of Oral Biosciences 54.1 (2012): 58-65.
