StemRNA - 3rd Gen Reprogramming Kit

Catalog 00-0076
Price
$1,295.00
$1,295.00
Availability In stock
Catalog
00-0076

Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine

The latest evolution in Stemgent RNA reprogramming combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
 

Key Benefits

  • Flexible technology generates high-quality human iPS cell lines from multiple target cell types
    Out-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs)
  • High efficiency, non-integrating reprogramming
    StemRNA - 3rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, prividing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs).
  • Time-saving protocol delivers faster results facilitating higher throughput
    Colonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
  StemRNA - 3rd Gen (00-0076)
Feature FibroblastsUrine (UPCs)Blood (EPCs)
No. wells per kit 9 3 3
No Transfections required 4 6-8 6-8
Days to primary iPS cell colonies 10-14 12-14 12-14
Reprogramming efficiency 2-+4% 0.1-0.5% 0.4-3%
Screening Required No No No
Xeno-compatible protocol Yes No No
GMP-compatible RNA manufacturing protocol Yes Yes Yes

 

P7 primary iPS cells derived from UPCs were stained using the StainAlive™ TRA-1-60 Antibody (09-0068) and DAPI after expansion in NutriStem™ hPSC XF Medium (01-0005)

iPS cells derived from fibroblasts using the StemRNA - 3rd Gen Reprogramming Kit were cultured on iMatrix-511 (NP892-011) in NutriStem™ hPSC XF Medium (01-0005) for 7 passages. Magnification: 4x

Timeline for StemRNA-NM reprogramming of fibroblasts

Download our StemRNA - 3rd Gen Reprogramming Posters

Poster Thumbnail
ISSCR 2015

ISSCR 2016 Poster
ISSCR 2016


Product Specifications

Product Name
StemRNA - 3rd Gen Reprogramming Kit
Catalog Number
00-0076
Storage and Stability
Store all three kit components at or below -70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
Quality Control
The individual mRNAs are tested for size and integrity. The StemRNA - 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for mycoplasma.
Recommended Usage
For use with associated StemRNA-NM protocols for generation of iPS cells.
Kit Contents
  • OKSMNL NM-RNA (Part No. 05-0040), 30 μg, 1 vial
  • EKB NM-RNA (Part No. 05-0041), 22 μg, 1 vial
  • NM-microRNAs (Part No. 05-0042), 15 μg, 1 vial


Product Specifications

Product Name
StemRNA - 3rd Gen Reprogramming Kit
Catalog Number
00-0076
Storage and Stability
Store all three kit components at or below -70 °C. Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
Quality Control
The individual mRNAs are tested for size and integrity. The StemRNA - 3rd Gen Reprogramming Kit is functionally validated for successful RNA-based reprogramming of adult fibroblasts, human umbilical vein endothelial cells (HUVECs), EPCs, and UPCs. Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. All components of the kit are sterile and have tested negative for mycoplasma.
Recommended Usage
For use with associated StemRNA-NM protocols for generation of iPS cells.
Kit Contents
  • OKSMNL NM-RNA (Part No. 05-0040), 30 μg, 1 vial
  • EKB NM-RNA (Part No. 05-0041), 22 μg, 1 vial
  • NM-microRNAs (Part No. 05-0042), 15 μg, 1 vial