Stemolecule Y27632

Catalog 04-0012
04-0012-10
Price
$125.00
$125.00
Availability In stock
Catalog
04-0012-base

Product Overview

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor1. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors3. Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation4.

Y27632 is widely used in naïve stem cell protocols5, such as the 5i/L/A/ protocol.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naïve pluripotent stem cell applications.


Product Specifications

Product Name
Stemolecule Y27632
Catalog Number
04-0012-base
Alternate Name(s)
Y-27632
(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
Chemical Formula
C14H21N3O·2HCl
Molecular Weight
320.3
CAS Number
146986-50-7
Purity
Greater than 97% by HPLC analysis
Formulation
White solid
Solubility
Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability
Store powder at 4 °C protected from light. Following reconstitution, store aliquots at -20 °C. Stock solutions are stable for 6 months when stored as directed.
Quality Control
The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.


References

  1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
  2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
  3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
  4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.
  5. Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naïve human pluripotent stem cells. World journal of stem cells, 7(3), 649 (2015).

Additional Publications

Product Specifications

Product Name
Stemolecule Y27632
Catalog Number
04-0012-base
Alternate Name(s)
Y-27632
(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
Chemical Formula
C14H21N3O·2HCl
Molecular Weight
320.3
CAS Number
146986-50-7
Purity
Greater than 97% by HPLC analysis
Formulation
White solid
Solubility
Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability
Store powder at 4 °C protected from light. Following reconstitution, store aliquots at -20 °C. Stock solutions are stable for 6 months when stored as directed.
Quality Control
The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.

References

  1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
  2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
  3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
  4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.
  5. Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naïve human pluripotent stem cells. World journal of stem cells, 7(3), 649 (2015).

Additional Publications