Stemolecule PD173074

Catalog 04-0008
Size 2 mg
Price
$119.00
$119.00
Availability In stock
Catalog
04-0008

Product Overview

Stemolecule PD173074 is a potent, cell-permeable ATP-competitive inhibitor of fibroblast growth factor (FGF) receptor1. Inhibition of the FGF signaling pathway by PD173074 leads to self-renewal of stem cells in many systems, including mouse and human2,3. PD173074 has also been found to suppress tumor growth in a wide variety of cancers through it’s interaction with FGF receptors4,5


Product Specifications

Product Name
Stemolecule PD173074
Catalog Number
04-0008
Size
2 mg
Alternate Name(s)
1-t-Butyl-3-(6-(3,5- dimethoxyphenyl)-2- (4-diethylaminobutylamino)- pyrido[2,3-d]pyrimidin- 7-yl)urea
Chemical Formula
C28H41N7O3
Molecular Weight
523.7
CAS Number
219580-11-7
Purity
Greater than 98% by HPLC analysis
Formulation
Yellow solid
Solubility
For a 10 mM concentrated stock solution of PD173074, reconstitute the compound by adding 381.9 μL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light.
Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at -20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of PD173074 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of PD173074 was tested on HeLa and HEK293 cells.

Specification Sheets

Safety Data Sheets


References

  1. Mohammadi, M., Froum, S., Hamby, J.M., Schroeder, M.C., Panek, R.L., Lu, G.H., Eliseenkova, A.V., Green, D., Schlessinger, J., and Hubbard, S.R. (1998) Crystal structure of an angiogenesis inhibitor bound to the FGF receptor tyrosine kinase domain. EMBO J. 17: 5896-5904.
  2. Zaragosi, L.E., Ailhaud, G., Dani, C. (2006) Autocrine fibroblast growth factor 2 signaling is critical for self-renewal of human multipotent adipose-derived stem cells. Stem Cells 24: 2412-2419.
  3. Ying, Q.L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., and Smith, A. (2008) The ground state of embryonic stem cell self renewal. Nature 453: 519-523.
  4. Dimitroff, C.J., Klohs, W., Sharma, A., Pera, P., Driscoll, D., Veith, J., Steinkempf, R., Schroeder, M., Klutchko, S., Sumlin, A., Henderson, B., Dougherty, T.J, and Bernacki, R.J. (1999) Anti-angiogenic activity of selected receptor tyrosine kinase inhibitors, PD166285 and PD173074: implications for combination treatment with photodynamic therapy. Invest New Drugs 17: 121-135.
  5. St Bernard, R., Zheng, L., Liu, W., Winer, D., Asa, S.L., and Ezzat, S. (2005) Fibroblast growth factor receptors as molecular targets in thyroid carcinoma. Endocrinology 147: 1145-1153.

Additional Publications

Product Specifications

Product Name
Stemolecule PD173074
Catalog Number
04-0008
Size
2 mg
Alternate Name(s)
1-t-Butyl-3-(6-(3,5- dimethoxyphenyl)-2- (4-diethylaminobutylamino)- pyrido[2,3-d]pyrimidin- 7-yl)urea
Chemical Formula
C28H41N7O3
Molecular Weight
523.7
CAS Number
219580-11-7
Purity
Greater than 98% by HPLC analysis
Formulation
Yellow solid
Solubility
For a 10 mM concentrated stock solution of PD173074, reconstitute the compound by adding 381.9 μL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light.
Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at -20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of PD173074 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of PD173074 was tested on HeLa and HEK293 cells.

Specification Sheets

Safety Data Sheets

References

  1. Mohammadi, M., Froum, S., Hamby, J.M., Schroeder, M.C., Panek, R.L., Lu, G.H., Eliseenkova, A.V., Green, D., Schlessinger, J., and Hubbard, S.R. (1998) Crystal structure of an angiogenesis inhibitor bound to the FGF receptor tyrosine kinase domain. EMBO J. 17: 5896-5904.
  2. Zaragosi, L.E., Ailhaud, G., Dani, C. (2006) Autocrine fibroblast growth factor 2 signaling is critical for self-renewal of human multipotent adipose-derived stem cells. Stem Cells 24: 2412-2419.
  3. Ying, Q.L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., and Smith, A. (2008) The ground state of embryonic stem cell self renewal. Nature 453: 519-523.
  4. Dimitroff, C.J., Klohs, W., Sharma, A., Pera, P., Driscoll, D., Veith, J., Steinkempf, R., Schroeder, M., Klutchko, S., Sumlin, A., Henderson, B., Dougherty, T.J, and Bernacki, R.J. (1999) Anti-angiogenic activity of selected receptor tyrosine kinase inhibitors, PD166285 and PD173074: implications for combination treatment with photodynamic therapy. Invest New Drugs 17: 121-135.
  5. St Bernard, R., Zheng, L., Liu, W., Winer, D., Asa, S.L., and Ezzat, S. (2005) Fibroblast growth factor receptors as molecular targets in thyroid carcinoma. Endocrinology 147: 1145-1153.

Additional Publications