Stemolecule IM-12

Catalog 04-0081
Size 2 mg
Price
$84.00
$84.00
Availability In stock
Catalog
04-0081

Product Overview

The indolylmaleimide IM-12 is a potent enzymatic (IC50= 52 nM, compared with 92 nM for SB-216763) and intracellular inhibitor of GSK-3β which activates downstream components of canonical Wnt signaling and increasing β-catenin levels. IM-12 promotes increased differentiation of neural progenitor cells to neurons1. IM-12 has been shown to support the conversion and maintenance of human naïve stem cells2.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplment necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor™ LIF, Human Recombinant (03-0016), and

A: Stemfactor™ Activin A, Human Recombinant (03-0001)


Product Specifications

Product Name
Stemolecule IM-12
Catalog Number
04-0081
Size
2 mg
Alternate Name(s)
3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione
Chemical Formula
C22H20FN3O2
Molecular Weight
377.41
CAS Number
1129669-05-1
Purity
99% Purity by HPLC analysis
Solubility
IM-12 is soluble in DMSO at 100 mM and in ethanol at 10 mM at room temperature. For a 10 mM concentrated stock solution of IM-12, reconstitute the compound by adding 530 μL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. The maximum concentration recommended for dilution in media to ensure complete solubility is 10 μM For cell culture, the media should be pre-warmed prior to adding the reconstituted compountd. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume.
Storage and Stability
Store powder for up to six months at 4 °C, protected from light.
Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at -20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of IM-12 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of IM-12 was tested on mouse embryonic stem cells.
Appearance
Orange solid

Specification Sheets

Safety Data Sheets


Product Specifications

Product Name
Stemolecule IM-12
Catalog Number
04-0081
Size
2 mg
Alternate Name(s)
3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione
Chemical Formula
C22H20FN3O2
Molecular Weight
377.41
CAS Number
1129669-05-1
Purity
99% Purity by HPLC analysis
Solubility
IM-12 is soluble in DMSO at 100 mM and in ethanol at 10 mM at room temperature. For a 10 mM concentrated stock solution of IM-12, reconstitute the compound by adding 530 μL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. The maximum concentration recommended for dilution in media to ensure complete solubility is 10 μM For cell culture, the media should be pre-warmed prior to adding the reconstituted compountd. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume.
Storage and Stability
Store powder for up to six months at 4 °C, protected from light.
Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at -20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
Quality Control
The purity of IM-12 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of IM-12 was tested on mouse embryonic stem cells.
Appearance
Orange solid

Specification Sheets

Safety Data Sheets