TRA-1-81 Antibody (Purified), Mouse anti-Human

Catalog 09-0011
Size 100 μL
Price
$109.00
$109.00
Availability In stock
Catalog
09-0011

Product Overview

The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embyronal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

ICC of H1 cells with TRA-1-81 antibody

ICC of H1 cells with TRA-1-81 antibody

Flow cytometry on H1 cells using TRA-1-81 antibody

ICC analysis of TRA-1-81 on H1 human ES cells at 20X magnification. Cells were stained with TRA-1-81 Antibody (Purified) at a 1:100 dilution followed by a secondary DyLight 488 conjugated antibody (green). DAPI staining was performed to visualize nuclei (blue).

Immunocytochemistry analysis of TRA-1-81 on H1 human ES cells. Cells were stained with TRA-1-81 Antibody (Purified) at a 1:100 dilution followed by a secondary Cy™ 3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

Flow cytometry of TRA-1-81 on H1 human ES cells. Purple histogram represents TRA-1-81 Antibody (Purified) and open histogram represents isotype control at same concentration.


Product Specifications

Product Name
TRA-1-81 Antibody (Purified), Mouse anti-Human
Catalog Number
09-0011
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 μg per 1 x 106 viable cells in 100 μL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.
Concentration
0.5 mg/mL
Isotype
Mouse IgM, κ
Immunogen
Human embryonal carcinoma cell line 2102Ep
Reactivity
Human
Preparation
This antibody was purified by affinity chromatography.
Clone
Tra-1-81


References

  1. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
  4. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.

Additional Publications

Product Specifications

Product Name
TRA-1-81 Antibody (Purified), Mouse anti-Human
Catalog Number
09-0011
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 μg per 1 x 106 viable cells in 100 μL for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunofluorescence Staining of Cells for Flow Cytometry.
Concentration
0.5 mg/mL
Isotype
Mouse IgM, κ
Immunogen
Human embryonal carcinoma cell line 2102Ep
Reactivity
Human
Preparation
This antibody was purified by affinity chromatography.
Clone
Tra-1-81

References

  1. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.
  2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.
  3. Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., and Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
  4. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.

Additional Publications