Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog 09-0024
Size 100 μL
Price
$182.00
$182.00
Availability In stock
Catalog
09-0024

Product Overview

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated embryonic stem (ES) cells. Chromatin immunoprecipitation experiments have shown that Sox2 and Oct4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development. The knockdown of Sox2 gene expression by siRNA in human ES cells results in loss of the undifferentiated stem cell state. Sox2 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. The Sox2 Antibody (Affinity Purified) was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best antibody available for researchers needing to demonstrate pluripotency.

Immunocytochemistry analysis of Sox2 on R1 mouse ES cells. Cells were stained with Sox2 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy™3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).


Product Specifications

Product Name
Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Catalog Number
09-0024
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, 1% BSA, and 0.1% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry staining to ensure product quality.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.
Concentration
0.5 mg/mL
Isotype
Rabbit IgG
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acid 45 and 261 of human Sox2
Reactivity
Mouse, Human
Preparation
This antibody was purified by antigen affinity chromatography.
Clone
Polyclonal


References

  1. Rodda, D.J., Chew, J.L., Lim, L.H., Loh, Y.H., Wang, B., Ng, H.H., and Robson
  2. Boyer, L.A., Lee, T.I., Cole, M.F., Johnstone, S.E., Levine, S.S., Zucker, J.P., Guenther, M.G., Kumar, R.M., Murray, H.L., Jenner, R.G., Gifford, D.K., Melton, D.A., Jaenisch, R., and Young, R.A. (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122: 947-956.
  3. Fong, H., Hohenstein, K.A., and Donovan, P.J. (2008) Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cell 26: 1931-1938.
  4. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
  5. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  6. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

Additional Publications

  1. Paull D; Sevilla A; Zhou H; Hahn AK; Kim H; Napolitano C; Tsankov A; Shang L; Krumholz K; Jagaseesan P; Woodard CM; Sun B; Vilboux T; Zimmer M; Forero E; Moroziewicz DN; Martinez H; Malicdan MCV; Weiss KA; Vensand LB; Dusenberry CR; Polus H; Sy KTL; Kahler DJ; Gahl WA; Solomon SL; Chang S; Meissner A; Eggan K; Noggle SA. "Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells." Naure Methods 12:885 (2015)
  2. Han F; Wang W; CHen B; CHen C; LI Sen; Lu X; Duan J; Zhang Y; Zhang YA; Guo W; Li G. "Human induced pluripotent stem cell-derived neurons improve motor asymmetry in a 6-hydroxydopamine-induced rat model of Parkinson's disease." Cytotherapy 17:665 (2015)

Product Specifications

Product Name
Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Catalog Number
09-0024
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, 1% BSA, and 0.1% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry staining to ensure product quality.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunoflourescence Staining of Cells for Flow Cytometry.
Concentration
0.5 mg/mL
Isotype
Rabbit IgG
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acid 45 and 261 of human Sox2
Reactivity
Mouse, Human
Preparation
This antibody was purified by antigen affinity chromatography.
Clone
Polyclonal

References

  1. Rodda, D.J., Chew, J.L., Lim, L.H., Loh, Y.H., Wang, B., Ng, H.H., and Robson
  2. Boyer, L.A., Lee, T.I., Cole, M.F., Johnstone, S.E., Levine, S.S., Zucker, J.P., Guenther, M.G., Kumar, R.M., Murray, H.L., Jenner, R.G., Gifford, D.K., Melton, D.A., Jaenisch, R., and Young, R.A. (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122: 947-956.
  3. Fong, H., Hohenstein, K.A., and Donovan, P.J. (2008) Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Stem Cell 26: 1931-1938.
  4. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
  5. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  6. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

Additional Publications

  1. Paull D; Sevilla A; Zhou H; Hahn AK; Kim H; Napolitano C; Tsankov A; Shang L; Krumholz K; Jagaseesan P; Woodard CM; Sun B; Vilboux T; Zimmer M; Forero E; Moroziewicz DN; Martinez H; Malicdan MCV; Weiss KA; Vensand LB; Dusenberry CR; Polus H; Sy KTL; Kahler DJ; Gahl WA; Solomon SL; Chang S; Meissner A; Eggan K; Noggle SA. "Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells." Naure Methods 12:885 (2015)
  2. Han F; Wang W; CHen B; CHen C; LI Sen; Lu X; Duan J; Zhang Y; Zhang YA; Guo W; Li G. "Human induced pluripotent stem cell-derived neurons improve motor asymmetry in a 6-hydroxydopamine-induced rat model of Parkinson's disease." Cytotherapy 17:665 (2015)