Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human

Catalog 09-0023
Size 100 μL
Price
$197.00
$197.00
Availability In stock
Catalog
09-0023

Product Overview

Oct4 (Octamer-4, also known as POU5F1) is a homeodomain transcription factor of the POU family which is involved in the regulation of pluripotency during normal development. The expression of Oct4 is associated with an undifferentiated phenotype in embryonic stem (ES) cells, while gene knockdown of Oct4 promotes differentiation. As such, it is frequently used as a marker for undifferentiated ES cells or induced pluripotent stem (iPS) cells. Oct4 is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. TheOct4 Antibody (Affinity Purified)was screened on human and mouse ES cells using immunocytochemistry and flow cytometry and selected as the best Oct4 antibody available for researchers needing to demonstrate pluripotency.

Oct4 staining of human ES cells

Immunocytochemistry analysis of Oct4 on H1 human ES cells. Cells were treated with Oct4 Antibody (Affinity Purified) using a 1:100 dilution followed by a secondary Cy™3 conjugated antibody (red). DAPI staining was performed to visualize nuclei (blue).

FACS Analysis of Oct4-stained H1 cells

Flow cytometry analysis of Oct4 on H1 human ES cells. Purple histogram represents Oct4 Antibody and open histogram represents isotype control at the same concentration.


Product Specifications

Product Name
Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Catalog Number
09-0023
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.4, 1% BSA and 0.02% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Related Products
mRNA Reprogramming Kit (00-0071)
microRNA Booster Kit (00-0073)
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunocytochemistry.
Isotype
Rabbit IgG
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Oct4
Reactivity
Mouse, Human
Preparation
The antibody was purified by immunogen affinity chromatography.
Clone
Polyclonal


References

  1. Looijenga, L.H., Stoop, H., de Leeuw, H.P., de Gouveia Brazao, C.A., Gillis, A.J., van Roozendaal, K.E., van Zoelen, E.J., Weber, R.F., Wolffenbuttel, K.P., van Dekken, H., Honecker, F., Bokemeyer, C., Perlman, E.J., Schneider, D.T., Kononen, J., Sauter, G., Oosterhuis, J.W. (2003) POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63: 2244-2250.
  2. Zaehres, H., Lensch, M.W., Daheron, L., Stewart, S.A., Itskovitz-Eldor, J., Daley, G.Q. (2005) High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23: 299-305.
  3. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
  4. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  5. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

Additional Publications

Product Specifications

Product Name
Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human
Catalog Number
09-0023
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.4, 1% BSA and 0.02% sodium azide
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry and flow cytometry to ensure product quality.
Related Products
mRNA Reprogramming Kit (00-0071)
microRNA Booster Kit (00-0073)
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and a 1:5 dilution for flow cytometry. For an application specific protocol, please see Protocol: Immunocytochemistry and Protocol: Immunocytochemistry.
Isotype
Rabbit IgG
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of human Oct4
Reactivity
Mouse, Human
Preparation
The antibody was purified by immunogen affinity chromatography.
Clone
Polyclonal

References

  1. Looijenga, L.H., Stoop, H., de Leeuw, H.P., de Gouveia Brazao, C.A., Gillis, A.J., van Roozendaal, K.E., van Zoelen, E.J., Weber, R.F., Wolffenbuttel, K.P., van Dekken, H., Honecker, F., Bokemeyer, C., Perlman, E.J., Schneider, D.T., Kononen, J., Sauter, G., Oosterhuis, J.W. (2003) POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res 63: 2244-2250.
  2. Zaehres, H., Lensch, M.W., Daheron, L., Stewart, S.A., Itskovitz-Eldor, J., Daley, G.Q. (2005) High-efficiency RNA interference in human embryonic stem cells. Stem Cells 23: 299-305.
  3. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676.
  4. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
  5. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K., and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134: 877-886.

Additional Publications