Stemfactor TGF-β1, Human Recombinant

Catalog 03-0004
Size 5 μg
Price
$225.00
$225.00
Availability In stock
Catalog
03-0004

Product Overview

Transforming Growth Factor beta 1 (TGF-β1) is a member of the TGF-β superfamily of multifunctional polypeptide growth factors that includes bone morphogenetic proteins (BMPs), Activin, and Nodal. TGF-β1 both helps maintain pluripotency in human embryonic stem (hES) cells and induces differentiation1,2. It also induces epithelial-mesenchymal transition and suppresses proliferation and differentiation of several types of lymphocytes, thus playing important roles in human cancers3. Stemfactor TGF-β1 is a 25 kDa homodimeric recombinant protein expressed in human HEK293 cells and composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.


Product Specifications

Product Name
Stemfactor TGF-β1, Human Recombinant
Catalog Number
03-0004
Size
5 μg
Purity
Greater than 95% by SDS-PAGE analysis
Formulation
Lyophilized from sterile-filtered 200 mM NaCl, 50 mM NaOAc, pH 4.5.
Reconstitution
Centrifuge briefly and then reconstitute TGF-β1 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/mL. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Stemfactor TGF-β1 is shipped at room temperature. Lyophilized TGF-β1 is stable for up to 6 months from date of receipt when stored at -20 °C to -80 °C. Reconstituted TGF-β1, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at -20 °C and up to 6 months when stored at -80 °C.
Source
Stemfactor TGF-β1 was expressed in and purified from human HEK293 cells.
Amino Acid Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Uniprot Accession Number
P01137, residues 279-390.
Endotoxin Level
Less than 1.0 EU/μg of TGF-β1 as determined by the LAL method.
Biologic Activity
The ED50 is less than 0.2 ng/mL as determined by its ability to inhibit the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells.

Specification Sheets

Safety Data Sheets


Product Specifications

Product Name
Stemfactor TGF-β1, Human Recombinant
Catalog Number
03-0004
Size
5 μg
Purity
Greater than 95% by SDS-PAGE analysis
Formulation
Lyophilized from sterile-filtered 200 mM NaCl, 50 mM NaOAc, pH 4.5.
Reconstitution
Centrifuge briefly and then reconstitute TGF-β1 in 4 mM HCl to yield a stock solution of no less than 0.1 mg/mL. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Stemfactor TGF-β1 is shipped at room temperature. Lyophilized TGF-β1 is stable for up to 6 months from date of receipt when stored at -20 °C to -80 °C. Reconstituted TGF-β1, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at -20 °C and up to 6 months when stored at -80 °C.
Source
Stemfactor TGF-β1 was expressed in and purified from human HEK293 cells.
Amino Acid Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Uniprot Accession Number
P01137, residues 279-390.
Endotoxin Level
Less than 1.0 EU/μg of TGF-β1 as determined by the LAL method.
Biologic Activity
The ED50 is less than 0.2 ng/mL as determined by its ability to inhibit the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells.

Specification Sheets

Safety Data Sheets