Stemfactor LIF, Human Recombinant

Catalog 03-0016
03-0016-100
Size 1 mL, 10 μg/mL
1 mL, 100 μg/mL
Price
$225.00
$225.00
Availability In stock
Catalog
03-0016-base

Product Overview

Recombinant Human LIF is a lymphoid factor that promotes long-term maintenance of the pluripotency of embryonic stem cells by suppressing spontaneous differentiation1,2. Leukemia Inhibitory Factor (LIF) has additional functions such as cholinergic neuron differentiation, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo3. Human LIF is an approximate 20 kDa protein containing 202 amino acid residues and is active on both human and mouse cells.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor™ LIF, Human Recombinant (03-0016), and

A: Stemfactor™ Activin A, Human Recombinant (03-0001)


Product Specifications

Product Name
Stemfactor LIF, Human Recombinant
Catalog Number
03-0016-base
Size
1 mL, 10 μg/mL
1 mL, 100 μg/mL
Purity
Greater than 99% by SDS-PAGE analysis.
Formulation
LIF is supplied as a 0.22 µm sterile filtered liquid in PBS containing 1% w/v BSA as carrier.
Storage and Stability
Store LIF at 4 °C. Do not freeze. Stable for up to 6 months from date of receipt, when stored as directed.
Concentration

1 mL, 10 μg/mL (Cat. No. 03-0016)
1 mL, 100 μg/mL (Cat. No. 03-0016-100)

Source
E. coli
Endotoxin Level
Less than 1 EU/μg of LIF as determined by the LAL method.
Biologic Activity
Human LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1x106 units/mL (Cat. No. 03-0016) and 1x107 units/mL (Cat. No. 03-0016-100), where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium.


Product Specifications

Product Name
Stemfactor LIF, Human Recombinant
Catalog Number
03-0016-base
Size
1 mL, 10 μg/mL
1 mL, 100 μg/mL
Purity
Greater than 99% by SDS-PAGE analysis.
Formulation
LIF is supplied as a 0.22 µm sterile filtered liquid in PBS containing 1% w/v BSA as carrier.
Storage and Stability
Store LIF at 4 °C. Do not freeze. Stable for up to 6 months from date of receipt, when stored as directed.
Concentration

1 mL, 10 μg/mL (Cat. No. 03-0016)
1 mL, 100 μg/mL (Cat. No. 03-0016-100)

Source
E. coli
Endotoxin Level
Less than 1 EU/μg of LIF as determined by the LAL method.
Biologic Activity
Human LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1x106 units/mL (Cat. No. 03-0016) and 1x107 units/mL (Cat. No. 03-0016-100), where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium.