Stemfactor FGF-basic, Human Recombinant

Catalog 03-0002
Size 50 μg
Price Price on Request
$137.00
Availability In stock
Catalog
03-0002

Product Overview

Fibroblast Growth Factor-basic (bFGF), also known as FGF-2, is a heparin-binding member of the FGF superfamily of molecules. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. Additionally, bFGF is a critical component of embryonic stem cell culture medium, allowing cells to remain in an undifferentiated state in serum-free medium1,2. Stemfactor bFGF is an approximate 17 kDa protein consisting of 155 amino acid residues. 


Product Specifications

Product Name
Stemfactor FGF-basic, Human Recombinant
Catalog Number
03-0002
Size
50 μg
Purity
Greater than 95% by SDS-PAGE analysis
Formulation
Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.
Reconstitution
Centrifuge briefly and then reconstitute bFGF in 500 μL of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/mL of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at -20 °C to -80 °C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at -20 °C and up to 6 months when stored at -80 °C.
Sterility
Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.
Source
E. coli
Amino Acid Sequence
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Uniprot Accession Number
P09038, residues 1-155
Endotoxin Level
Less than 1.0 EU/μg of bFGF as determined by the LAL method.
Biologic Activity
The ED50 is 0.1 to 1 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.

Specification Sheets

Safety Data Sheets

Application Notes


References

  1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thompson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278.
  2. Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O’Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323.
  3. Vallier, L., Alexander, M., and Pedersen, R.A. (2005) Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495-4509.
  4. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.
  5. Greber, B., Lehrach, H., and Adjaye, J. (2007) Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells 25: 455-464.

Additional Publications

Product Specifications

Product Name
Stemfactor FGF-basic, Human Recombinant
Catalog Number
03-0002
Size
50 μg
Purity
Greater than 95% by SDS-PAGE analysis
Formulation
Lyophilized from 5 mM Tris-HCl, pH 7.5 with 150 mM NaCl.
Reconstitution
Centrifuge briefly and then reconstitute bFGF in 500 μL of 10 mM Tris, pH 7.6, to yield a stock solution of 0.1 mg/mL of bFGF. Avoid freeze-thaw cycles as it can result in loss of activity.
Storage and Stability
Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at -20 °C to -80 °C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at -20 °C and up to 6 months when stored at -80 °C.
Sterility
Tested to be negative for Mycoplasma sp. by PCR and microbial contamination by a sterility test.
Source
E. coli
Amino Acid Sequence
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Uniprot Accession Number
P09038, residues 1-155
Endotoxin Level
Less than 1.0 EU/μg of bFGF as determined by the LAL method.
Biologic Activity
The ED50 is 0.1 to 1 ng/mL as determined by the dose dependent proliferation of NIH 3T3 cells.

Specification Sheets

Safety Data Sheets

Application Notes

References

  1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thompson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271-278.
  2. Xu, C., Rosler, E., Jiang, J., Lebkowski, J.S., Gold, J.D., O’Sullivan, C., Delavan-Boorsma, K., Mok, M., Bronstein, A., and Carpenter, M.K. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323.
  3. Vallier, L., Alexander, M., and Pedersen, R.A. (2005) Activin/Nodal and FGF pathways corporate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495-4509.
  4. Wang, G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D., and Deng, H. (2005) Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330: 934-942.
  5. Greber, B., Lehrach, H., and Adjaye, J. (2007) Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal. Stem Cells 25: 455-464.

Additional Publications