StainAlive SSEA-1 Antibody (DyLight 488), Mouse anti-Mouse/Human

Catalog 09-0067
Size 100 μL
Price
$279.00
$279.00
Availability In stock
Catalog
09-0067

Product Overview

Stage-specific embryonic antigen-1 (SSEA-1), also known as CD15, is an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma (EC) cells, murine embryonic stem (ES) cells, and murine and human germ cells. SSEA-1 is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration, and differentiation. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers do not change after staining with this antibody.

R1 Mouse ESCs stained with 09-0067

Immunocytochemical analysis of SSEA-1 on R1 mouse ES cells. Phase contrast view of cells in culture and the same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 488) using a 1:100 dilution.

       

Product Specifications

Product Name
StainAlive SSEA-1 Antibody (DyLight 488), Mouse anti-Mouse/Human
Catalog Number
09-0067
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, containing no preservative
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see General Protocol for ICC Staining of Live Cells”.
Concentration
0.5 mg/mL
Isotype
Mouse IgM, κ
Immunogen
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity
Mouse, Human
Preparation
This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 μm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus.
Clone
MC-480


References

  1. Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159.
  2. Fox, N., Damjanov, I., Martinez-Hernandez, A., Knowles, B.B., and Solter, D. (1981) Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol 83: 391-398.
  3. Andrews, P.W., Goodfellow, P.N., Shevinsky, L., Bronson, D.L., and Knowles, B.B. (1982) Cell surface antigens of a clonal human embryonic carcinoma cell line: morphological and antigenic differentiation in culture. Int. J. Cancer 29: 523-531.
  4. Draper, J.S., Pigott, C., Thomson, J.A., and Andrews, P.W. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J. Anat. 200: 249-258.
  5. Cui, L., Johkura, K., Yue, F., Ogiwara, N., Okouchi, Y., Asanuma, K., and Sasaki, K. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J Histochem Cytochem 52: 1447-1457.

Additional Publications

Product Specifications

Product Name
StainAlive SSEA-1 Antibody (DyLight 488), Mouse anti-Mouse/Human
Catalog Number
09-0067
Size
100 μL
Formulation
Phosphate-buffered solution, pH 7.2, containing no preservative
Storage and Stability
Store at 4 °C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Quality Control
Tested by immunocytochemistry to ensure product quality. Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay.
Recommended Usage
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please see General Protocol for ICC Staining of Live Cells”.
Concentration
0.5 mg/mL
Isotype
Mouse IgM, κ
Immunogen
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity
Mouse, Human
Preparation
This antibody was purified by affinity chromatography and conjugated with DyLight 488 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. 0.2 μm filter sterilized. Formulation is free from bacteria, fungi, mycoplasma, and mouse virus.
Clone
MC-480

References

  1. Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159.
  2. Fox, N., Damjanov, I., Martinez-Hernandez, A., Knowles, B.B., and Solter, D. (1981) Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol 83: 391-398.
  3. Andrews, P.W., Goodfellow, P.N., Shevinsky, L., Bronson, D.L., and Knowles, B.B. (1982) Cell surface antigens of a clonal human embryonic carcinoma cell line: morphological and antigenic differentiation in culture. Int. J. Cancer 29: 523-531.
  4. Draper, J.S., Pigott, C., Thomson, J.A., and Andrews, P.W. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J. Anat. 200: 249-258.
  5. Cui, L., Johkura, K., Yue, F., Ogiwara, N., Okouchi, Y., Asanuma, K., and Sasaki, K. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J Histochem Cytochem 52: 1447-1457.

Additional Publications