Stemolecule Wnt Inhibitor IWP-3 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1. The inhibition of the Wnt pathway through the use of IWP-3 has also been shown to promote the formation of cardiomyocytes from human embryonic stem cell-derived mesoderm cells2.
Stemolecule Wnt Inhibitor IWP-3
Greater than 99% by HPLC analysis
Light yellow solid
This molecule is soluble in DMSO at 100 mM.
For a 10 mM concentrated stock solution of Wnt Inhibitor IWP-3, reconstitute the compound by adding 412.7 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, prewarm the medium prior to adding reconstituted compound. Note: For most cell culture applications, the total DMSO concentration should be kept below 0.5%.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of Wnt Inhibitor IWP-3 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-3 was tested on mouse embryonic stem cells.