The aminopyrimidinyl carbamate Stemolecule WH-4-023 is a potent Src and LCK inhibitor (IC50< 10 nM).1 WH-4-023 has been shown to support the conversion to and maintenance of human naive pluripotent stem cells.2
Stemgent conveniently provides all the components comprising the 5i/L/A media supplements necessary to support naive pluripotent stem cell applications.
L: Stemfactor LIF, Human Recombinant (03-0016), and
A: Stemfactor Activin A, Human Recombinant (03-0001)
Greater than 99% purity by HPLC analysis
Off-white to brown color solid
This molecule is soluble in DMSO at 100 mM and in ethanol at 2.5 mM at room temperature.
For a 10 mM concentrated stock solution of WH-4-023, reconstitute the compound by adding 352 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be pre-warmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume.
Storage and Stability
Store powder for up to six months at 4 °C, protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of WH-4-023 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of WH-4-023 was tested on mouse embryonic stem cells.