Stemolecule Valproic Acid (VPA) is a cell-permeable, small molecule that has been shown to affect several pathways. VPA is a histone deacetylase (HDAC) inhibitor which improves reprogramming efficiency by at least 100 fold1. VPA also has been found to affect both the Extracellular Signal-Regulated Kinase (ERK), Protein Kinase C (PKC), and the Wnt/β-Catenin pathways. VPA has been reported to regulate the differentiation and proliferation of various cells, including mesenchymal and hematopoietic stem cells, neuroblastoma cells, primary neurons and neural progenitor cells (NPCs)2. In the case of hepatic differentiation of mouse embryonic stem cells, Valproic Acid in combination with cytokines differentiate cells into a uniform and homogeneous cell population of hepatic progenitor cells followed by maturation into functional hepatocytes3.
Stemolecule Valproic Acid
Greater than 98% by gas chromatography
White crystalline solid
This molecule is reported to be soluble in DMSO at 100 mM and water at 300 mM.
For a 10 mM stock solution of Valproic Acid, add 3.01 mL DMSO to 5 mg of the compound. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding VPA.
Storage and Stability
Store powder at 4 °C protected from light. Following reconstitution, store aliquots at −20 °C. Stock solutions are stable for 6 months when stored as directed.
The purity of VPA was determined by gas chromatography. The accurate mass was determined by mass spectrometry. Cellular toxicity of VPA was tested on mouse embryonic stem cells.