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Stemgent Stemolecule Purmorphamine

Code: 04-0009-base
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Purmorphamine is a small molecule that promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts1,2. The mechanism of action study of Purmorphamine indicates that it is an agonist of Smoothened, a 7-transmembrane receptor of the hedgehog signaling pathway3,4. Purmorphamine can also be used to replace sonic hedgehog for the generation of motor neurons from human embryonic stem cells5.

Product Name

Stemolecule Purmorphamine

Catalog Number

04-0009

Size

5 mg

Alternate Name(s)

2-(1-Naphthoxy)-6-(4-morpholinoanilino)- 9-cyclohexylpurine

Chemical Formula

C31H32N6O2

Molecular Weight

520.6

CAS Number

483367-10-8

Purity

Greater than 96% by HPLC analysis

Formulation

Pale beige solid

Solubility

For a 10 mM concentrated stock solution of Purmorphamine, reconstitute the compound by adding 960.4 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For use in cell culture, warm the medium just prior to adding the reconstituted compound. Once the compound is added, mix and filter-sterilize the medium using a 0.2 uM low-protein binding filter. Purmorphamine is soluble in DMSO at 50 mM.

Storage and Stability

Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control

The purity of Purmorphamine was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM — 100 uM of Purmorphamine.

Specification Sheets

Safety Data Sheets

  1. Wu, X., Ding, S., Ding, Q., Gray, N.S., and Shultz, P.G. A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J Am Chem Soc 124: 14520-14521 (2002).
  2. Beloti, M.M., Bellesini, L.S., and Rosa, A.L. Puromorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells. Cell Biol Int 29: 537-541 (2005).
  3. Wu, X., Walker, J., Zhang, J., Ding, S., and Schultz, P.G. Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem Biol 11: 1229-1238 (2004).
  4. Sinha, S., and Chen, J.K. Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat Chem Biol 2: 29-30 (2006).
  5. Li, X.J., Hu, B.Y., Jones, S.A., Zhang, Y.S., Lavaute, T., Du, Z.W., and Zhang, S.C. Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules. Stem Cells 26: 886-893 (2008).

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