The indolylmaleimide IM-12 is a potent enzymatic (IC50= 52 nM, compared with 92 nM for SB-216763) and intracellular inhibitor of GSK-3β which activates downstream components of canonical Wnt signaling and increasing β-catenin levels. IM-12 promotes increased differentiation of neural progenitor cells to neurons1. IM-12 has been shown to support the conversion and maintenance of human naive stem cells2.
Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naive pluripotent stem cell applications.
L: Stemfactor LIF, Human Recombinant (03-0016), and
A: Stemfactor Activin A, Human Recombinant (03-0001)
99% by HPLC analysis
IM-12 is soluble in DMSO at 100 mM and in ethanol at 10 mM at room temperature. For a 10 mM concentrated stock solution of IM-12, reconstitute the compound by adding 530µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. The maximum concentration recommended for dilution in media to ensure complete solubility is 10 uM For cell culture, the media should be pre-warmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume.
Storage and Stability
Store powder for up to six months at 4 °C, protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of IM-12 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of IM-12 was tested on mouse embryonic stem cells.