Stemolecule Forskolin is a natural compound produced by the Indian Coleus plant (Coleus forskohlii)1. It is used in several differentiation protocols for its ability to potentiate neuron differentiation2,3. Forskolin is able to stimulate adenylate cyclase activity and increases cyclic AMP4. Cyclic AMP is a signaling molecule and key regulator of critical enzymes in cellular processes5. For example, cAMP can bind to protein kinase A (PKA) regulatory subunit and activate PKA which acts as a negative regulator of the hedgehog signaling pathway6.
Greater than 97% by HPLC analysis
White crystalline powder
For a 10 mM concentrated stock solution of Forskolin, reconstitute the compound by adding 2.44 mL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of Forskolin was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Forskolin was tested on mouse embryonic stem cells.