Stemgent Stemolecule BIO

Code: 04-0003-base
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BIO (6-bromoindirubin-3'-oxime) is a potent, reversible and ATP-competitive inhibitor for GSK-3α/β in the Wnt signaling pathway. BIO is the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells1. BIO-mediated activation of the Wnt signaling cascade maintained the undifferentiated state of both mouse and human embryonic stem cells under feeder free conditions possibly by Wnt-induced increase in the expression of Nodal2-4.

Product Name

Stemolecule BIO

Catalog Number

04-0003

Size

2 mg

Alternate Name(s)

(2'Z,3'E)-5-Bromoindirubin-3'-oxime

Chemical Formula

C16H10BrN3O2

Molecular Weight

356.17

CAS Number

667463-62-9

Purity

Greater than 98% by HPLC analysis

Formulation

Dark red solid

Solubility

For a 10 mM concentrated stock solution of BIO, reconstitute the compound by adding 561.5 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is reported to be soluble in DMSO at 100 mM and ethanol at 10 mM.

Storage and Stability

Store powder at 4 °C or −20 °C, protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C. The effect of storage of stock solutions should be verified for each application.

Quality Control

The purity of BIO was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of BIO was tested on mouse embryonic stem cells.

Specification Sheets

Safety Data Sheets

  1. Sato, N., Meijer, L., Skaltsounis, L., Greengard, P., and Brivanlou, A.H. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10: 55-63 (2004).
  2. Besser, D. Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires activation of Smad 2/3. J Biol Chem 279: 45076-45084 (2004).
  3. Ying, Q.L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., and Smith, A. The ground state of embryonic stem cell self renewal. Nature 453: 519-523 (2008).
  4. Polychronopoulos, P., Magiatis, P., Skaltsounis, A.L., Myrianthopoulos, V., Mikros, E., Tarricone, A., Musacchio, A., Roe, S.M., Pearl, L., Leost, M., Greengard, P., and Meijer, L. Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J Med Chem 47: 935-946 (2004).

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