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Stemgent Stemolecule Y27632

Code: 04-0012-base
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Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor1. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors3. Y27632 in combination with Pifithrin-u significantly improves cell recovery after cryopreservation4.

Stemolecule Y27632 is also available as a 10 mM DMSO Stock Solution (Cat. No. 04-0012-02).

Y27632 is widely used in naive stem cell protocols5, such as the 5i/L/A/ protocol.

Stemgent conveniently provides all the components comprising the 5i/L/A media supplement necessary to support naive pluripotent stem cell applications.

5i (inhibitors):

L: Stemfactor LIF, Human Recombinant (03-0016), and

A: Stemfactor Activin A, Human Recombinant (03-0001)

Product Name

Stemolecule Y27632

Catalog Number

04-0012

Size

2 mg (Cat. No. 04-0012)

10 mg (Cat. No. 04-0012-10)

2 mg in 10 mM DMSO (Cat. No. 04-0012-02)

Alternate Name(s)

  • Y-27632
  • (1R,4R)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride

Chemical Formula

C14H21N3O·2HCl

Molecular Weight

320.3

CAS Number

146986-50-7

Purity

Greater than 97% by HPLC analysis

Formulation

White solid

Solubility

Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.

Storage and Stability

Store powder at 4 °C protected from light. Following reconstitution, store aliquots at −20 °C. Stock solutions are stable for 6 months when stored as directed.

Quality Control

The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.

  1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983 (2000).
  2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686 (2007).
  3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86: 270-280 (2008).
  4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog 26: 781-788 (2010).
  5. Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naive human pluripotent stem cells. World Journal of Stem Cells, 7(3), 649 (2015).

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