Stemolecule R(+)Bay K 8644 is an L-type Ca2+ channel activator1,2. This molecule has recently been demonstrated to enhance reprogramming efficiency. When only two transcription factors (Oct4 and Klf4) are introduced into mouse embryonic fibroblasts (MEFs), and when used in combination with a histone methyltransferase G9a inhibitor BIX01294 (Cat. No. 04-0002), R(+)Bay K 8644 enhances the efficiency of iPS cell generation significantly3.
Stemolecule R(+)Bay K 8644
(4R)-1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-trifluoromethyl)phenyl]-3-pyridinecarboxylic acid methyl ester
Greater than 99% by HPLC analysis
For a 10 mM concentrated stock solution of R(+)Bay K 8644, reconstitute the compound by adding 1.4 mL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of R(+)Bay K 8644 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of R(+)Bay K 8644 was tested on mouse embryonic stem cells.