Stemolecule IDE-1 was identified in a high throughput screen as an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells. IDE-1 is able to generate Sox17+/FoxA2+ cells to an equal or greater efficiency than that of protein (i.e. Activin A or Nodal) IDEs in mouse or human ES cell cultures. It functions in part via activation of TGFβ signaling, as evident by Smad2 phosphorylation. IDE-1 chemically induced endoderm can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos1.
Greater than 95% by HPLC analysis
For a 10 mM concentrated stock solution of IDE-1, reconstitute the compound by adding 652.9 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is reported to be soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of IDE-1 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of IDE-1 was tested on mouse embryonic stem cells.