Stemolecule GSK429286A is a cell-permeable, selective small molecule inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK)1. ROCKs are serine/threonine specific protein kinases which play a crucial role in organization of the actin cytoskeleton2. ROCK inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2,3. Due to GSK429286AÕs specificity, this reagent may be useful in assessing the cellular roles of individual ROCK isoforms.
Greater than 99% by HPLC analysis
For a 10 mM concentrated stock solution of GSK429286A, reconstitute the compound by adding 462.6 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is reported to be soluble in DMSO at 100 mM.
Storage and Stability
Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.
The purity of GSK429286A was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of GSK429286A was tested on mouse embryonic stem cells.