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Stemgent Stemolecule AM580

Code: 04-0077-base
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AM580 is a highly specific small molecule agonist for retinoic acid receptor α (RARα). Retinoids are known for their affect on a number of pathways including those related to cell growth, differentiation, and apoptosis1. Specifically, AM580 has been shown to induce differentiation in leukemic cell lines and inhibit angiogenesis in chick embryos2,3. More recently, Liu et al. demonstrated the significance of retinoic acid (RA) signaling in reprogramming somatic cells to induced pluripotent stem (iPS) cells. Treatment of mouse embryonic fibroblasts (MEFs) with AM580 early in the reprogramming process significantly increases reprogramming efficiency up to 100 fold4. This suggests promoting RA signaling with AM580 may have important implications in reprogramming to a ground-state pluripotency (naive stem cells).

Product Name

Stemolecule AM580

Catalog Number

04-0077

Size

5 mg

Alternate Name(s)

4-[(5,6,7,8-Tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)carboxamido]benzoic acid

Chemical Formula

C22H25NO3

Molecular Weight

351.44

CAS Number

102121-60-8

Purity

Greater than 95% by HPLC analysis

Formulation

White crystalline solid

Solubility

For a 10 mM concentrated stock solution of AM580, reconstitute the compound by adding 1.42 mL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 50 mM.

Storage and Stability

Store powder at either 4 °C or −20 °C, protected from light. Information about stability of Stemolecules in solution is not available. As a general rule, we recommend following reconstitution, store aliquots at −20 °C. Stability of stock solutions should be validated in each application.

Quality Control

The purity of AM580 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of AM580 was tested on mouse embryonic stem cells.

Specification Sheets

Safety Data Sheets

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