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Stemgent Stemolecule A83-01

Code: 04-0014-base
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A83-01 is a selective inhibitor of the transforming growth factor-β (TGFβ) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the nodal receptor ALK71. This molecule is more potent than SB431542 in its inhibition of ALK4, 5, and 7, and only weakly inhibits ALK1, 2, 3, and 6. A83-01 inhibits the TGFβ-induced epithelial-to-mesenchymal transition (EMT) via the inhibition of Smad2 phosphorylation2.

Product Name

Stemolecule A83-01

Catalog Number

04-0014

Pack Size

2 mg (Cat. No. 04-0014)

10 mg (Cat. No. 04-0014-10)

Alternate Name(s)

  • A-83-01
  • A 83-01
  • 3-(6-Methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide

Chemical Formula

C25H19N5S

Molecular Weight

421.52

CAS Number

909910-43-6

Purity

Greater than 98% by HPLC analysis

Formulation

Pale yellow solid

Solubility

A83-01 is soluble in DMSO at 50 mM.

Storage and Stability

Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C. The effect of storage of stock solutions should be verified for each application.

Quality Control

The purity of A83-01 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. No acute cytotoxicity was observed in mouse embryonic stem cells following a 6 hour exposure to 1 nM - 100 µM A83-01.

  1. Tojo, M., Hamashima, Y., Hanyu, A., Kajimoto, T., Saitoh, M., Miyazono, K., Node, M., and Imamura, T. The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factorβ. Cancer Sci 96: 791-800 (2005).
  2. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H., and Ding, S. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4: 16-19 (2009).

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