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Stemgent mRNA Reprogramming Factors Set: hOKSML

Stemgent mRNA Reprogramming Factors Set: hOKSML

Code: 00-0067-base
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Stemgent mRNA Reprogramming Factors Set: hOKSML contains mRNA encoding Oct4, Klf4, Sox2, c-Myc, Lin-28, and nuclear GFP (nGFP). These in vitro transcribed mRNAs incorporate both pseudouridine and 5-methylcytidine-modified nucleotides to minimize the cellular interferon response to single stranded RNA.1,2 Repeated transfection of mRNA encoding Oct4, Klf4, Sox2, c-Myc and Lin-28 has been shown to reprogram human fibroblasts.2 nGFP mRNA is included for use as a positive control for RNA transfection during the cellular reprogramming process.

Advantages

  • Faster reprogramming, colonies emerge as early as 16 days
  • Stoichiometric control of individual reprogramming factors
  • Eliminates need for backend screening of viral systems
  • Validated protocol using normal and diseased cells

Morphological timeline for mRNA iPS Colony Formation. Day 1 of chronological morphology changes during the emergence of a primary iPS colony generated using StemgentÕs mRNA Reprogramming Factor Set on ParkinsonÕs disease patient dermal fibroblasts.

Morphological timeline for mRNA iPS Colony Formation. Day 16 of chronological morphology changes during the emergence of a primary iPS colony generated using StemgentÕs mRNA Reprogramming Factor Set on ParkinsonÕs disease patient dermal fibroblasts.

Morphological timeline for mRNA iPS Colony Formation. Day 20 of chronological morphology changes during the emergence of a primary iPS colony generated using StemgentÕs mRNA Reprogramming Factor Set on ParkinsonÕs disease patient dermal fibroblasts.

Pluripotent human iPS cell colonies generated with the mRNA Reprogramming System. Phase contrast image of an mRNA-derived iPS cell colony. Immunohistochemistry image of same colony stained for the pluripotency markers. (Nanog/Tra-1-60)

Pluripotent human iPS cell colonies generated with the mRNA Reprogramming System. Phase contrast image of an mRNA-derived iPS cell colony. Immunohistochemistry image of same colony stained for the pluripotency markers. (Oct4/Tra-1-81)

Pluripotent human iPS cell colonies generated with the mRNA Reprogramming System. Phase contrast image of an mRNA-derived iPS cell colony. Immunohistochemistry image of same colony stained for the pluripotency markers. (Rex1/SSEA4)

Relative endogenous mRNA expression level comparison for Nanog and Oct4. qRT-PCR calculations and data normalization against HuES9 p62 human ES positive control cell line.

Product Name

mRNA Reprogramming Factors Set: hOKSML

Catalog Number

00-0067

Formulation

100ng/ul in RNase-free TE buffer, pH 7.0

Storage and Stability

Store at or below −70°C. Stable for a minimum of 3 months from date of receipt when stored as directed. Wear gloves when handling this product. Use RNase-free tubes and barrier pipette tips. Thaw on ice. Keep on ice until the sample is removed. Avoid multiple freezing and thawing cycles (< 5). Aliquot to experimental quantities.

Quality Control

Proper protein expression and localization have been confirmed by immunocytochemistry for reprogramming factors and by fluorescence microscopy for nGFP.

Related Products

  • Stemgent Oct4 mRNA, human (Cat.No.05-0014 )
  • Stemgent Klf4 mRNA, Human (Cat.No.05-0015)
  • Stemgent Sox2 mRNA, Human (Cat.No.05-0016)
  • Stemgent c-Myc mRNA, Human (Cat.No.05-0018)
  • Stemgent Lin-28 mRNA, Human (Cat.No.05-0017)
  • Stemgent nGFP mRNA (Cat.No.05-0019)
  • Stemfect RNA Transfection Kit (Cat.No.00-0069)
  • Stemolecule Y27632 (Cat.No.04-0012)
  • Stemfactor Fibroblast Growth Factor-basic (Human Recombinant) (Cat.No.03-0002)
  • mRNA Reprogramming Kit (00-0071)
  • microRNA Booster Kit (00-0073)

Recommended Usage

mRNAs should be transfected using a protocol specifically optimized for the cell type used in the experiment.

Kit Contents

  • 2 vials — Stemgent Oct4 mRNA, Human (Cat. No. 05-0014), 20µg
  • 1 vial — Stemgent Klf4 mRNA, Human (Cat. No. 05-0015), 20µg
  • 1 vial — Stemgent Sox2, Human (Cat. No. 05-0016), 20µg
  • 1 vial — Stemgent c-Myc mRNA, Human (Cat. No. 05-0018), 20µg
  • 1 vial — Stemgent Lin-28 mRNA, Human (Cat. No. 05-0017), 20µg
  • 1 vial — Stemgent nGFP mRNA (Cat. No. 05-0019), 20µg
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