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Alvetex Publications: 101 and up (newest)

A list of Publications in Scientific Journals detailing research where Alvetex™ has been used.

The publications below are hosted on the websites of various scientific journals.



Mechanistic analysis and significance of sphingomyelinasemediated decreases in transepithelial CFTR currents in nHBEs
Kirsten A. Cottrill, Vincent D. Giacalone, Camilla Margaroli, Robert J. Bridges, Michael Koval, Rabindra Tirouvanziam, Nael A. McCarty

Physiological Reports. 2021; 9:e15023
DOI: 10.14814/phy2.15023


Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells
Giedre Kvist

Methods in Molecular Biology (MIMB), volume 2352. First online 30 July 2021
DOI: 10.1007/978-1-0716-1601-7_17


Barrier-on-a-Chip with a Modular Architecture and Integrated Sensors for Real-Time Measurement of Biological Barrier Function
Patrícia Zoio, Sara Lopes-Ventura and Abel Oliva

Micromachines 2021, 12(7), 816 (12 July 2021)


Pigmented full-thickness human skin model based on a fibroblast-derived matrix for long-term studies
Mrs. Patrícia Zoio, Mrs. Sara Ventura, Mrs. Mafalda Leite, and Dr. Abel Oliva

Tissue Engineering Part C: Methods, 19 Jun 2021


miR-378d regulates polyploidization and malignant phenotype of tumor cells through AKT and RhoA
Jie Peng, Susu Shi, Juan Yu, Jianli Liu, Haixiang Wei, Haixia Song, Shaoqiang Wang, Daolu Guo, Zhejie Li, Shujin He, Lei Li, Hongyan Zhang, Zhizhen Yan, Ran Zhao, Yukun Liu, Yanrong Liu, Junjun Li, Renya Zhang, Wei Wang

Preprints 2021, 2021060443 (16 June 2021)
doi: 10.20944/preprints202106.0443.v1


Immune modulatory effects of Idelalisib in stromal cells of chronic lymphocytic leukemia
Sarah Handl, Franziska von Heydebrand, Simon Voelkl, Robert A. J. Oostendorp, Jochen Wilke , Anita N. Kremer, Andreas Mackensen, and Gloria Lutzny-Geier

Leukemia & Lymphoma, 17 May 2021


Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts
L. A. Smith, A. Hidalgo Aguilar, D. D. G. Owens, R. H. Quelch, E. Knight and S. A. Przyborski

Frontiers in Cell Development and Biology, 06 May 2021

Abstract: Various methods are currently used to investigate human tissue differentiation, including human embryo culture and studies utilising pluripotent stem cells (PSCs) such as in vitro embryoid body formation and in vivo teratoma assays. Each method has its own distinct advantages, yet many are limited due to being unable to achieve the complexity and maturity of tissue structures observed in the developed human. The teratoma xenograft assay allows maturation of more complex tissue derivatives, but this method has ethical issues surrounding animal usage and significant protocol variation. In this study, we have combined three-dimensional (3D) in vitro cell technologies including the common technique of embryoid body (EB) formation with a novel porous scaffold membrane, in order to prolong cell viability and extend the differentiation of PSC derived EBs. This approach enables the formation of more complex morphologically identifiable 3D tissue structures representative of all three primary germ layers. Preliminary in vitro work with the human embryonal carcinoma line TERA2.SP12 demonstrated improved EB viability and enhanced tissue structure formation, comparable to teratocarcinoma xenografts derived in vivo from the same cell line. This is thought to be due to reduced diffusion distances as the shape of the spherical EB transforms and flattens, allowing for improved nutritional/oxygen support to the developing structures over extended periods. Further work with EBs derived from murine embryonic stem cells demonstrated that the formation of a wide range of complex, recognisable tissue structures could be achieved within 2–3 weeks of culture. Rudimentary tissue structures from all three germ layers were present, including epidermal, cartilage and epithelial tissues, again, strongly resembling tissue structure of teratoma xenografts of the same cell line. Proof of concept work with EBs derived from the human embryonic stem cell line H9 also showed the ability to form complex tissue structures within this system. This novel yet simple model offers a controllable, reproducible method to achieve complex tissue formation in vitro. It has the potential to be used to study human developmental processes, as well as offering an animal free alternative method to the teratoma assay to assess the developmental potential of novel stem cell lines.


Extracellular Vesicles From a Three-Dimensional Culture of Perivascular Cells Accelerate Skin Wound Healing in a Rat
Min Ho Kim, Changho Chung, Mun Ho Oh, Jin Hyun Jun, Yong Ko & Jong Hun Lee

Aesthetic Plastic Surgery, 05 April 2021


SIRT1 Inhibits Apoptosis by Promoting Autophagic Flux in Human Nucleus Pulposus Cells in the Key Stage of Degeneration via ERK Signal Pathway
Fei He, Qingshu Li, Bo Sheng, Haitao Yang, and Wei Jiang

BioMed Research International, vol. 2021, Article ID 8818713.


Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro
Costello L, Darling N, Freer M, Bradbury S, Mobbs C, Przyborski S

Methods in Molecular Biology (Clifton, N.J.), 1 January 2021
DOI: 10.1007/978-1-0716-1246-0_20

Abstract: In vitro epithelial models are valuable tools for both academic and industrial laboratories to investigate tissue physiology and disease. Epithelial tissues comprise the surface epithelium, basement membrane, and underlying supporting stromal cells. There are various types of epithelial tissue and they have a diverse and intricate architecture in vivo, which cannot be successfully recapitulated using two-dimensional (2D) cell culture. Tissue engineering strategies can be applied to bioengineer the organized, multilayered, and multicellular structure of epithelial tissues in vitro. Alvetex® is a porous, polystyrene scaffold that enables fibroblasts to synthesize a complex network of endogenous, humanized extracellular matrix proteins. This creates a physiologically relevant three-dimensional (3D) subepithelial microenvironment, enriched with mechanical and chemical cues, which supports the organization and differentiation of epithelial cells. Such technology has been used to bioengineer different epithelial architectures in vitro, including the simple, columnar structure of the intestine and the stratified, squamous, and keratinized structure of skin. Epithelial tissue models provide a useful platform for fundamental and translational research, with multifaceted applications including disease modeling, drug discovery, and product development.


Generation of Keratinocytes from Human Induced Pluripotent Stem Cells Under Defined Culture Conditions
Shyam Kishor Sah, Jitendra K. Kanaujiya, I-Ping Chen, and Ernst J. Reichenberger

Cellular Reprogramming, 29 December 2020
DOI: 10.1089/cell.2020.0046


The human bone marrow harbors a CD45CD11B+ cell progenitor permitting rapid microglia‐like cell derivative approaches
Andreas Bruzelius Isabel Hidalgo Antonio Boza‐Serrano Anna‐Giorgia Hjelmér Amelie Tison Tomas Deierborg Johan Bengzon Tania Ramos‐Moreno

Stem Cells Translational Medicine, 09 December 2020
DOI: 10.1002/sctm.20-0127


Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures
Claudia Consales, Alessio Butera, Caterina Merla, Emanuela Pasquali, Vanni Lopresto, Rosanna Pinto, Maria Pierdomenico, Mariateresa Mancuso, Carmela Marino & Barbara Benassi

Molecular Neurobiology, 24 November 2020


Developing a novel 3D model of the intestinal epithelium as a tool to study the pharmacokinetics of new chemical entities
Matthew A. Freer

Doctoral Thesis, Durham University, UK
25 September 2020


Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function
Nicole J. Darling, Claire L. Mobbs, Ariana L. González-Hau1, Matthew Freer and Stefan Przyborski

Frontiers in Bioengineering and Biotechnology
31 August 2020


E7 oncoprotein from human papillomavirus 16 alters claudins expression and the sealing of epithelial tight junctions
Perla Yaceli Uc, Jael Miranda, Arturo Raya‑Sandino, Lourdes Alarcón, María Luisa, Roldán Rodolfo, Ocadiz‑Delgado, Enoc Mariano Cortés‑Malagón, Bibiana Chávez‑Munguía, Georgina Ramírez, René Asomoza, Liora Shoshani, Patricio Gariglio, Lorenza González‑Mariscal

International Journal of Oncology
29 July 2020
DOI: 10.3892/ijo.2020.5105


Spheroid Trapping and Calcium Spike Estimation Techniques toward Automation of 3D Culture
Kenneth Ndyabawe, Mark Haidekker, Amish Asthana, William S. Kisaalita

SLAS Technology
16 July 2020
DOI: 10.1177/2472630320938319