Alvetex Protocols

Alvetex Scaffold

Basics

Alvetex Scaffold: quick start	Download PDF
Media volumes guide for different feeding options in Alvetex Scaffold in well insert formats	Download PDF
Alvetex Scaffold 96 well plate: quick start	Download PDF
Alvetex Scaffold 96 well plate: instructions for use and example applications	Download PDF
Alvetex Scaffold 384 well plate: quick start	Download PDF
Alvetex Scaffold 384 well plate: instructions for use and example application	Download PDF

Coating

Poly-D or L-lysine coating of Alvetex Scaffold	Download PDF
Collagen I coating of Alvetex Scaffold	Download PDF
Collagen I thin gel coating of Alvetex Scaffold	Download PDF
Matrigel® coating of Alvetex Scaffold	Download PDF
Fibronectin coating of Alvetex Scaffold	Download PDF

Cell-Specific

Guidance notes and protocols for the culture of primary rat hepatocytes in Alvetex Scaffold in well insert and well plate formats	Download PDF
Protocol for the culture of primary rat hepatocytes in Alvetex Scaffold 96 well plate format	Download PDF
Culture of the canine kidney epithelial MDCK cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocol for the culture of the MCF-10A cell line on Alvetex Scaffold (12 well insert format and 96-well plate format)	Download PDF
Example protocol for the culture of the CaCo2 cell line on Alvetex Scaffold in well iInsert and well plate formats with or without a collagen I gel layer	Download PDF
Example protocol for the co-culture of the Caco-2 cell line with the CCD-18co cell line on Alvetex Scaffold in well insert formats	Download PDF
Example protocol for the culture of the breast carcinoma MDA-MB-231 cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocol for the culture of the human colon fibroblastic CCD-18co cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocol for the culture of the lung carcinoma A549 cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocol for the culture of the breast invasive ductal carcinoma BT-474 cell line on Alvetex Scaffold	Download PDF
Example protocol for the culture of the PC-3 cell line on Alvetex Scaffold in well plate and well insert formats	Download PDF
Example protocol for the culture of the LN-229 cell line on Alvetex Scaffold in 96 well plate format	Download PDF
Example protocol for the culture of the 3T3 cell line on Alvetex Scaffold in well plate and well insert formats	Download PDF
Example protocol for the culture of the TERA2.cl.SP12 cell line on Alvetex Scaffold	Download PDF
Example protocol for the culture of the HepG2 cell line on Alvetex Scaffold in well plate and well insert formats	Download PDF
Example protocols for the Culture of the CHO-K1 cell line on Alvetex Scaffold in well plate and well insert formats	Download PDF
Example preparation of HaCaT epidermal equivalent using Alvetex Scaffold	Download PDF
Development of full thickness human skin model using Alvetex Scaffold technology	Download PDF
Example protocol for the Culture of the SW480 cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocol for the Culture of the SW620 cell line on Alvetex Scaffold in well insert and well plate formats	Download PDF
Example protocols for the co-culture of either the SW620 or the SW480 cell line with the 3T3 cell line on Alvetex Scaffold in well insert formats	Download PDF
Example protocol for the culture of the MG63 cell line on Alvetex Scaffold in well insert formats	Download PDF
Example protocol for the culture of primary rat MSCs on Alvetex Scaffold in well insert formats	Download PDF
Example protocols for the culture of the MCF-7 cell line on Alvetex Scaffold in well plate and well insert formats	Download PDF

Transfection

3D Transfection System (Mirus)	Download PDF

Cell Visualization

Simple visualisation of cells on Alvetex Scaffold using light microscopy (Neutral Red-Methyl Blue dye)	Download PDF
Live cell imaging of 3D cultures grown on Alvetex Scaffold using confocal microscopy	Download PDF
Wheat germ agglutinin staining of 3D cultures grown on Alvetex Scaffold and Alvetex Strata	Download PDF

Metabolic Assays

alamarBlue® cell viability assay of upcyte® hepatocyte cultures grown on Alvetex Scaffold in 3D	Download PDF
Determination of cell number and growth characteristics within Alvetex Scaffold using the PicoGreen® assay	Download PDF
XTT cell viability assay for Alvetex Scaffold 96 well plate format	Download PDF
MTS cell viability assay of upcyte® hepatocyte cultures grown on Alvetex Scaffold in 3D	Download PDF
MTT cell viability assay of cultures grown on Alvetex Scaffold in 3D	Download PDF

Genomics and Proteomics

Total protein extraction from cells cultured in Alvetex Scaffold in 3D	Download PDF
Protein extraction from cells cultured in Alvetex Scaffold 96 well plate format	Download PDF
Extraction of RNA from cells cultured in Alvetex Scaffold in 3D	Download PDF
RNA extraction from cells cultured in Alvetex Scaffold 96 well plate format	Download PDF

Histology

Histology (1): Choosing the Right Fixative to Preserve 3D Cell Cultures	Download PDF
Histology (2): Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence	Download PDF
Histology (3): Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining	Download PDF
Histology (4): Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy	Download PDF
Histology (5): Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)	Download PDF

Advanced Microscopy

(See also Histology Series Part 5, above.)

Processing Alvetex Scaffold 3D cultures for cryosectioning	Download PDF
Immunofluorescence confocal microscopy of 3D cultures grown on Alvetex Scaffold	Download PDF

Cell Retrieval

Retrieval of cells from Alvetex Scaffold	Download PDF
Protocol for the removal of Alvetex discs from 6 well or 12 well inserts by unclipping the well insert base	Download PDF