ReproNeuro AD-Mutation Neurons

Catalog RCDN002N
Size 3x106 cells
Price
$800.00
$800.00
Availability In stock
Catalog
RCDN002N

Product Overview

ReproNeuro AD-Mutation Neurons (RCDN002N)

Human Brain Alzheimer's Disease Model Neurons derived by Transfection

  • Frozen as a suspension of individualized human progenitor cells
  • Prepared using the same manufacturing process as wild-type ReproNeuro
  • Constructed by transfection with a mutated (P117L) Presenilin 1(PS1) gene introduced into an iPS cell line
  • Rapidly differentiates into neurons during in vitro growth and maturation (~2 weeks)
  • Expresses multiple neuron-specific markers; indicating a mixed population of neuron types
  • Shows an increased ratio of amyloid beta (Abeta42 / Abeta40) peptides as observed in Alzheimer's disease brain pathology
  • ReproNeuro RCDN001N (Lot A) is an isogenic control (the identical iPS cell line prior to transfection)
  • Developed with iCeMS (Institute for Integrated Cell-Material Sciences, Kyoto, Japan)

ReproNeuro iPSC-derived neurons are delivered as a suspension of frozen single cells that retain structural and functional integrity, and high viability thanks to REPROCELL's proprietary cryopreservation technology.   Thawed and plated, ReproNeuro cells mature during 2-3 weeks of culture on surfaces treated with ReproNeuro Coat (RCDN201).

Schematic showing preparation of neural progenitor cells from human iPS cells which were transfected mutated (P117L) presenilin 1 gene


Product Specifications

Product Name
ReproNeuro AD-Mutation Neurons
Catalog Number
RCDN002N
Size
3x106 cells
Storage and Stability
Store in liquid nitrogen


Literature

  • Makoto Honda, Itsunari Minami, Norie Tooi, Nobuhiro Morone, Hisae Nishioka, Kengo Uemura, Ayae Kinoshita, John E. Heuser, Norio Nakatsuji, Kazuhiro Aiba. The modeling of Alzheimer's disease by the overexpression of mutant Presenilin 1 in human embryonic stem cells. Biochemical and Biophysical Research Communications 469, 3(2016):587-592

  • Sakurai K, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.”Nucleic Acids Res. (2010): Apr;38(7):e96

Reference

Sakurai K, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.”Nucleic Acids Res. (2010): Apr;38(7):e96.

Product Specifications

Product Name
ReproNeuro AD-Mutation Neurons
Catalog Number
RCDN002N
Size
3x106 cells
Storage and Stability
Store in liquid nitrogen

Literature

  • Makoto Honda, Itsunari Minami, Norie Tooi, Nobuhiro Morone, Hisae Nishioka, Kengo Uemura, Ayae Kinoshita, John E. Heuser, Norio Nakatsuji, Kazuhiro Aiba. The modeling of Alzheimer's disease by the overexpression of mutant Presenilin 1 in human embryonic stem cells. Biochemical and Biophysical Research Communications 469, 3(2016):587-592

  • Sakurai K, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.”Nucleic Acids Res. (2010): Apr;38(7):e96

Reference

Sakurai K, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.”Nucleic Acids Res. (2010): Apr;38(7):e96.