A breakthrough in the culture of Primary Human Hepatocytes
ReproHP Medium enables the robust culture of primary human hepatocytes, enhancing and prolonging the detection of most CYP450 detoxification enzymes. Boosted basal expression levels, enhanced induction levels and lasting enzyme activity in primary human hepatocytes are key effects of ReproHP medium.
Higher Cytochrome P450 expression levels
When cultured in ReproHP Medium and exposed to specific drugs, the basal and induced expression levels of key detoxification enzymes (CYP3A4, CYP1A2, CYP2B6) are significantly elevated in primary human hepatocytes.
*Experimental Notes: Reported expression values are normalized against the basal expression level of another “leading brand”. The data shown is from one lot of primary human hepatocytes (Life Technologies) taken at Day 4 in culture. Other human primary hepatocyte lots showed similar patterns of enhanced expression with ReproHP Medium™.
3D Culture Leads to Higher Performance
Culturing primary human hepatocytes on the Alvetex scaffold in ReproHP Medium™ further boosts CYP3A4 basal expression levels and induced levels after drug induction.