Alvetex 3D Cell Culture: Protocols


1. Alvetex Scaffold protocols

1.1. Alvetex Scaffold Workflow, Part 1: Preparation, Setup and Seeding

 

1.1.1. Basic Protocols

PR-SC-01: Quick Start Protocol – Alvetex Scaffold

PR-SC-56: Alvetex Well Inserts – Media Volumes Guide

High throughput multiwell plates:

PR-SC-02: Quick Start Protocol – Alvetex Scaffold 96-well plate

PR-SC-03: Alvetex Scaffold 96-well plate: Instructions for Use and Sample Applications

PR-SC-04: Quick Start Protocol – Alvetex Scaffold 384 well plate

PR-SC-05: Alvetex Scaffold 384-well plate: Instructions for Use and Example Application

 

1.1.2. Alvetex Coating

PR-SC-06: Poly-D or L-lysine Coating of Alvetex Scaffold

PR-SC-07: Collagen I Coating of Alvetex Scaffold

PR-SC-08: Collagen I Thin Gel Coating on Alvetex Scaffold

PR-SC-09: Matrigel™ Coating on Alvetex Scaffold

PR-SC-10: Fibronectin Coating on Alvetex Scaffold

 

1.1.3. Cell Specific Protocols

PR-SC-11: Guidance notes and Protocols for the culture of Primary rat hepatocytes in well insert and well plate Alvetex Scaffold formats

PR-SC-12: Protocol for the culture of Primary rat hepatocytes in Alvetex Scaffold 96-well plate format

PR-SC-13: Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-14: Culture of MCF-10A Cell Line on Alvetex Scaffold (12-well insert format, and 96-well plate format)

PR-SC-15: Culture of CaCo-2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel Layer

PR-SC-16: Example Protocol for the Co-Culture of the CaCo-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert Formats

PR-SC-17: Culture of Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-18: Culture of Human Colon Fibroblastic CCD-18Co Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-19: Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-20: Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma Co-Culture BT-474 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-21: Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

PR-SC-22: Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96 Well Plate Format

PR-SC-23: Example Protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

PR-SC-24: Example Protocols for the Culture of the TERA2.cl.SP12 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

PR-SC-25: Example Protocols for the Culture of the HepG2 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

PR-SC-26: Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats

PR-SC-27: Example Preparation of HaCat cells Epidermal Equivalent Using Alvetex Scaffold

PR-SC-28: Development of Full Thickness Human Skin Model Using Alvetex Scaffold Technology

PR-SC-29: Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-30: Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats

PR-SC-31: Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert Formats

PR-SC-32: Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert Formats

PR-SC-33: Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert Formats

PR-SC-34: Example Protocol for the Culture of the MCF-7 Cell Line on Alvetex Scaffold

 

1.1.4. Transfection

PR-SC-35: Gene Transfection in 3D – Mirus®

 

1.2. Alvetex Scaffold Workflow, Part 2: Culturing and Monitoring 3D Cell Growth

 

1.2.1. Cell Visualisation

PR-SC-36: Light Microscopy – NR-MB Dye

PR-SC-37: Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal Microscopy

PR-SC-38: Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata

 

1.2.2. Metabolic Assays

PR-SC-38: alamarBlue® Viability Assay

PR-SC-39: PicoGreen Cell Number Assay

PR-SC-40: XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate Format

PR-SC-41: MTS Viability Assay

PR-SC-42: MTT Viability Assay

 

1.3. Alvetex Scaffold Workflow, Part 3: Imaging, Harvesting and Downstream Analysis

 

1.3.1. Genomics and Proteomics

PR-SC-43: Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3D

PR-SC-44: Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format

PR-SC-45: Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3D

PR-SC-46: RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format

 

1.3.2. Histology

PR-SC-47: Histology Series Part 1. Choosing the Right Fixative to Preserve 3D Cell Cultures

PR-SC-48: Histology Series Part 2. Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence

PR-SC-49: Histology Series Part 3. Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining

PR-SC-50: Histology Series Part 4. Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy

PR-SC-51: Histology Series Part 5. Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)

 

1.3.3. Advanced Microscopy

See also Histology Series Part 5, above.

PR-SC-52: Processing Alvetex Scaffold 3D Cultures for Cryosectioning

PR-SC-53: Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex Scaffold

 

1.3.4. Cell Retrieval

PR-SC-54: Retrieval of Cells from Alvetex Scaffold

PR-SC-55: Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert base

 

2. Alvetex Strata protocols

 

PR-ST-01: Quick Start Protocol – Alvetex Strata

PR-ST-01: Retrieval of Cells from Alvetex Strata

PR-SC-57: Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata


Protocols