Alvetex®

製品分類 : 3D培養基材研究テーマ : 3次元培養・共培養, 癌, 薬物代謝試験

Alvetex<sup>®</sup>

簡便な操作で、生体内に近い3次元細胞培養を再現性高く実現

≪特徴≫

・ 均質な多孔構造がもたらす、一貫した、再現性の高いデータ取得が可能
・ in vivo環境を模した理想的な幾何学構造。
 in vivo環境において、多くの細胞は毛細血管から150-200μm以内に存在。
 Alvetex®の厚みは200μmであるため、全ての細胞はAlvtex®表面から100μm以内の距離に存在
・ 一般的な2Dプレートに用いられるポリスチレンを材料として用いており、
 2D培養時と同じ試薬を用いたスイッチが容易。新たな機器購入も不要
・ 長期培養に耐える品質の高い安定性
・ ご希望の培養方法、アッセイ期間に応じて、最適な製品を選択可能


≪概要≫

様々な規格の製品をご用意しておりますので、ご使用になる細胞やアッセイに応じて選択して頂くことが可能です。

Alvetex® Scaffoldマルチウェルプレートタイプ

  • 各種のスループットアッセイに対応可能な3次元培養プレート
  • 細胞機能評価、遺伝子発現解析、タンパク質発現解析に使用可能
  • シンプルな作業手順(細胞播種→培地添加→培養開始)
  • 全自動ハイスループットアッセイに対応(96ウェル、384ウェルプレート)
  • 低コストでの3次元培養が可能

Alvetex® Scaffoldウェルインサートタイプ

  • イメージング、細胞機能評価、遺伝子発現解析、タンパク質発現解析に使用可能
  • シンプルな作業手順(細胞播種→培地添加→培養開始)
  • 6ウェルプレート、12ウェルプレートに対応
  • 低コストでの3次元培養が可能

Alvetex® Scaffold潅流プレートタイプ

  • シンプルで低コストな潅流培養システムを実現
  • 2次元培養、Alvetex 3次元培養、ウェルインサート培養に対応
  • 一般的なペリスタポンプが使用可能
  • ルアーロックを採用

Alvetex® Strataウェルインサートタイプ

  • 継代が可能な新規3次元培養システム
  • イメージング、細胞機能評価、遺伝子発現解析、タンパク質発現解析に使用可能
  • シンプルな作業手順(細胞播種→培地添加→培養開始)
  • 未分化状態を維持した幹細胞の長期培養が可能


製品のリスト・価格

Alvetex®Scaffoldマルチウェルプレートタイプ

Alvetex®Scaffoldを底面に使用したマルチウェルプレート
滅菌、個別包装済み

品番 製品名 内容 希望小売価格(税抜)
AVP002-1 Alvetex®Scaffold
12 well plate
1 x 12 well plates ¥4,000
AVP002-10 10 x 12 well plates ¥20,000
AVP006-1 Alvetex®Scaffold
24 well plate
1 x 24 well plates ¥5,000
AVP006-10 10 x 24 well plates ¥25,000
AVP009-1 Alvetex®Scaffold
96 well plate
1 x 96 well plates ¥6,000
AVP009-10 10 x 96 well plates ¥30,000
AVP010-1 Alvetex®Scaffold
384 well plate
1 x 384 well plates ¥8,000
AVP0010-10 10 x 384 well plates ¥40,000

Alvetex®Scaffoldウェルインサートタイプ

6ウェルプレート、12ウェルプレートに装着可能なインサートタイプのAlvetex®Scaffold
滅菌、個別包装済み

品番 製品名 内容 希望小売価格(税抜)
AVP004-3 Alvetex®Scaffold
6 well insert
3×6 well inserts ¥2,000
AVP004-96 96×6 well inserts ¥30,000
AVP005-3 Alvetex®Scaffold
12 well insert
3×12 well inserts ¥2,000
AVP005-96 96×12 well inserts ¥30,000

Alvetex®Scaffoldウェルインサートホルダー

Alvetex®ウェルインサートタイプを装着できるホルダーとペトリディッシュのセット
付属のペトリディッシュでAlvetex®Scaffoldを使った3次元培養が可能
滅菌、個別包装済み

品番 製品名 内容 希望小売価格(税抜)
AVP015-1 Alvetex®Scaffold well insert holder and deep Petri-dish 1 units ¥2,500
AVP015-10 10 units ¥10,000

Alvetex®Scaffold潅流プレート

Alvetex® Scaffoldウェルインサートタイプを装着可能な潅流プレート(ポンプと管は含まれません)
滅菌、個別包装済み

品番 製品名 内容 希望小売価格(税抜)
AVP011-1 Perfusion plate pack of 1 plates and Luer locks ¥4,500
AVP011-10 pack of 10 plates and Luer locks ¥25,000

Alvetex®Strataウェルインサートタイプ

6ウェルプレート、12ウェルプレートに装着可能なインサートタイプのAlvetex®Strata
滅菌、個別包装済み

品番 製品名 内容 希望小売価格(税抜)
STP004-3 Alvetex®Strata 6 well inserts 3 x 6 well inserts ¥2,000
STP004-96 96 x 6 well inserts ¥30,000
STP005-3 Alvetex®Strata 12 well inserts 3 x 12 well inserts ¥2,000
STP005-96 96 x 12 well inserts ¥30,000

アプリケーションノート(英語)

論文・発表

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    The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells
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    The effects of microRNAs on human neural stem cell differentiation in two- and three-dimensional cultures
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    Small molecule phosphorescent probes for O2 imaging in 3D tissue models
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    Beyond Cell Death ? Antiapoptotic Bcl-2 Proteins Regulate Migration and Invasion of Colorectal Cancer Cells In Vitro
    Public Library of Science., PLoS ONE 8(10): e76446., DOI:10.1371/journal.pone.0076446
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    Effect of PEG Surface Conformation on Anticancer Activity and Blood Circulation of Nanoemulsions Loaded with Tocotrienol-Rich Fraction of Palm Oil
    The AAPS Journal (August 2013), DOI: 10.1208/s12248-013-9525-z,
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    Overlapping gene coexpression patterns in human medullary thymic epithelial cells generate self-antigen diversity
    Benedikt Brorse, and Bruno Kyewskia, PNAS. 26 August 2013. DOI: 10.1073,
  • Ruchi Sharma, Safia Z. Barakzai, Sarah E. Taylor, F. Xavier Donadeu
    Epidermal-like architecture obtained from equine keratinocytes in three-dimensional cultures
    Journal of Tissue Engineering and Regenerative Medicine., First published online 30 July 2013. DOI: 10.1002/term.1788,
  • Jessica M. Stiles, Clarissa Amaya, Steven Rains, Dolores Diaz, Robert Pham, James Battiste, Jaime F. Modiano, Victor Kokta, Laura E. Boucheron, Dianne C. Mitchell, Brad A. Bryan
    Targeting of Beta Adrenergic Receptors Results in Therapeutic Efficacy against Models of Hemangioendothelioma and Angiosarcoma
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    Characterization of liver specific functions of upcyte® hepatocytes grown in 3D and co-cultured with upcyte® endothelial cells
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    Oncogene, 2012, doi: 10.1038/ocn.2012.111
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    Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays
    Xenobiotica, 2012,doi: 10.3109/00498254.2012.675093
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    General Xenobiochemistry, October 2012, Vol. 42, No. 10 , Pages 939-956, DOI:10.3109/00498254.2012.675093,
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    Alvetex: Polystyrene scaffold technology for routine three dimensional cell culture.
    Methods in Molecular Biology, 695, 323-40.
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    Developments in three dimensional cell culture technology aimed at improving the accuracy of in vitroanalyses.
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    Effect of synthesis parameters on emulsion-templated porous polymer formation and evaluation for 3D cell culture scaffolds.
    Journal of Materials Chemistry, 17, 4088-4094.
  • Bokhari, M., Carnachan, R., Cameron, N.R., Przyborski, S.A. (2007).
    Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge.
    Journal of Anatomy, 211, 567-76.
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    Novel cell culture device enabling three-dimensional cell growth and improved cell function.
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    Tailoring the morphology of emulsion-templated porous polymers.
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    Growth of human stem cell-derived neurons on solid three dimensional polymers.
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    Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds.
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