SynFire® Induced Neuron Co-Culture Kit

Human SynFire® neurons and Astroglia for co-culture studies

Co-Culture kits contain GABAergic and Glutamatergic Neurons, Astroglia, and all of the media necessary to establish a co-culture model for functional neuron studies. Kits containing only GABAergic neurons, astroglia and media (Cat. No. NC1002-10), or only glutamatergic neurons, astroglia, and media (Cat. No. NC1001-10) are also available.

Co-Culture Kits are available in two configurations suitable for general culture (Cat. No. NC1010-1.5) or MEA assays (Cat. No. NC1010-7.5). Please see the specification for kit contents.

Advantages of SynFire iNs include:

• Real Human Biology
  These cells more closely resemble real human biology than alternatives such as common animal models or other iPSC-based systems. SynFire iNs provide better suitability to predict responses to test agents.
• Rapid and Homogeneous Maturation
  SynFire iNs develop mature synaptic network activity within 3-4 weeks. These iNs show synchronous bursting phenotypes similar to rodent primary cultures.
• Reliable, Robust, and Ready-to-Use Cultures
  This differentiation approach results in a highly defined in vitro system with lot-to-lot consistency that provides reproducible results.
• Flexible Modular System
  The user can control subtype seeding densities and ratios to customize cultures to specific projects. Custom kit configurations are available.

Videos: see the NeuCyte Webinar Series online.

NeuCyte company name and logo and SynFire brand name are the property of NeuCyte Inc., USA.

Kit Contents

SynFire Co-culture Kit (Basic)    (Cat. No. NC1010-1.5)

• Part No. 1001-1.5: Glutamatergic induced neurons (>1.5x10⁶ cells/vial)
• Part No. 1002-1.5: GABAergic induced neurons (>1.5x10⁶ cells/vial)
• Part No. 1003-1.5: Astroglia (> 1.5x10⁶ cells/vial)
• Part No. 2001-10: Seeding Basal Media (Enough for 10 mL)
• Part No. 2001S-10: Seeding Supplement (Enough for 10 mL)
• Part No. 2002-20: Short-term Basal Media (Enough for 20 mL)
• Part No. 2002S-20: Short-term Supplement (Enough for 20 mL)
• Part No. 2003-60: Long-term Basal Media (Enough for 60 mL)
• Part No. 2003S-60: Long-term Supplement (Enough for 60 mL)

SynFire Co-culture Kit (MEA)    (Cat. No. NC1010-7.5)

• Part No. 1001-7.5: Glutamatergic induced neurons (>7.5x10⁶ cells/vial)
• Part No. 1002-3.5: GABAergic induced neurons (>3.5x10⁶ cells/vial)
• Part No. 1003-3.5: Astroglia (> 3.5x10⁶ cells/vial)
• Part No. 2001-20: Seeding Basal Media (Enough for 20 mL)
• Part No. 2001S-20: Seeding Supplement (Enough for 20 mL)
• Part No. 2002-40: Short-term Basal Media (Enough for 40 mL)
• Part No. 2002S-40: Short-term Supplement (Enough for 40 mL)
• Part No. 2003-120: Long-term Basal Media (Enough for 120 mL)
• Part No. 2003S-120: Long-term Supplement (Enough for 120 mL)

Storage and Stability

Store in cells in liquid nitrogen (Astroglia) or -80°C freezer (iNs) immediately upon receipt. Store other reagents at the temperature indicated on the label.

This product is stable for at least 6 months from the date of receiving when stored as directed.

QC

Functional validation of maturation and neural network formation potential of iPSC derived induced neurons has been done on MEAs. All cells have been tested for chromosomal integrity and the absence of pathogens (i.e. mycoplasma, etc). Full characterization included in the Certificate of Analysis.

Restricted Use

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures.

User guide:

• SynFire Users Guide

Application note:

• Long-term functional characterization of SynFire® iNs and astrocytes co-cultured on MaxTwo Multi-Well High-Density Microelectrode Array [Application note]

Application protocols:

• SynFire® iN plating protocol and chemical long-term potentiation on MaxWell Biosystems Multi-Well High-Density Microelectrode Array (HD-MEA) [Protocol]
• Protocol for MED64 Presto 24-well Sakura MEA plates [Protocol]

Videos:

NeuCyte Webinar Series

Publications using these products 

• Ishibashi Y et al. Responses to antibiotics in human iPSC-derived neurons based on the clinical antibiotic associated encephalopathy classification. J. Toxicol. Sci. (2022).
• Zaer H et al. Non-Ablative Doses of Focal Ionizing Radiation Alters Function of Central Neural Circuits. Brain Stimulation (2022).
• Pediaditakis I et al. A Microengineered Brain-Chip to Model Neuroinflammation in Humans. iSCIENCE (2022).
• Saavedra L et al. Comparison of Acute Effects of Neurotoxic Compounds on Network Activity in Human and Rodent Neural Cultures. Toxicol Sci. (2021). 
• SosciaD et al. A flexible 3-dimensional micro-electrode array for in vitro brain models. Lab Chip (2020).
• Tukker A et al. Towards Animal-Free Neurotoxicity Screening: Applicability of hiPSC-Derived Neuronal Models for In Vitro Seizure Liability Assessment. ALTEX (2020).