Stemolecule™ Wnt Inhibitor IWP-3

04-0035

Brand: Stemolecule

Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity.

CAS Number: 687561-60-0
Alternate Name(s):
  • 2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide

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Product name Product code Pack size Price (USD) Price (GBP) Price (EUR)
Stemolecule™ Wnt Inhibitor IWP-3 04-0035 2 mg $ 120.00 £ 99.00 € 115.00

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Product Information

Stemolecule Wnt Inhibitor IWP-3 was identified in a high throughput screen for antagonists of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity. It also blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1. The inhibition of the Wnt pathway through the use of IWP-3 has also been shown to promote the formation of cardiomyocytes from human embryonic stem cell-derived mesoderm cells2.

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Product Name: Stemolecule Wnt Inhibitor IWP-3

Catalog Number: 04-0035

Size: 2 mg

Alternate Name(s): 2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide

Chemical Formula: C22H17FN4O2S3

Molecular Weight: 484.59

CAS Number: 687561-60-0

Purity: Greater than 99 % by HPLC analysis

Formulation: Light yellow solid

Solubility: This molecule is soluble in DMSO at 100 mM.

Reconstitution: For a 10 mM concentrated stock solution of Wnt Inhibitor IWP-3, reconstitute the compound by adding 412.7 µL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37 °C for 2 to 5 minutes. For cell culture, prewarm the medium prior to adding reconstituted compound. Note: For most cell culture applications, the total DMSO concentration should be kept below 0.5%.

Storage and Stability: Store powder at 4 °C protected from light. Information about the stability of Stemolecules in solution is largely not available. As a general guideline, we recommend that stock solution be freshly made and stored in aliquots at −20 °C, protected from light. The effect of storage of stock solutions should be verified for each application.

Quality Control: The purity of Wnt Inhibitor IWP-3 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Wnt Inhibitor IWP-3 was tested on mouse embryonic stem cells.

Specification Sheets:

Safety Data Sheets:

  1. Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol 5: 100-107 (2009).
  2. Willems, E., Spiering, S., Davidovics, H., Lanier, M., Xia, Z., Dawson, M., Cashman, J., and Mercola, M. Small-molecule inhibitors of the Wnt pathway potently promote cardiomyocytes from human embryonic stem cell-derived mesoderm. Circ Res 109: 360-364 (2011).

Additional Publications