iMatrix-511 Stem Cell Culture Substrate

Catalog NP892-011
NP892-012
Size 2x175 µg and 6x175 µg available
Price
$270.00
$270.00
Availability In stock
Catalog
NP892-01

Recombinant Laminin-511 E8-Fragments

iMatrix-511 is xeno-free, recombinant laminin-511 E8-fragments, produced in CHO-S cells. iMatrix is used with the StemRNA 3rd Gen Reprogramming Kit (00-0076) to create an ideal system for generating iPS cells from fibroblasts, blood, or urine.

iMatrix-511's features make it an ideal matrix for pluripotent stem cell culture:

  • Promotes greater cell adhesion than all other matrix proteins that have been tested
  • Xeno-free formulation / CHO-S cell bioproduction
  • Easy to use (liquid format)
  • E8-fragments retain integrin binding specificity and capacity, and display higher potency than natural Laminin-511

Laminin is localized to the basement membrane, and plays a key role in cell adhesion and proliferation. Laminin-511 (α5, β1 laminin) binds to integrin α6β1 to promote cell signaling. Laminin-511 provides an ideal matrix for the proliferation of a wide variety of cell types especially stem cells and iPS cells.

iPSCs cultured on iMatrix-511 in StemFit medium


Product Specifications

Product Name
iMatrix-511 Stem Cell Culture Substrate
Catalog Number
NP892-01
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-511 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin α6β1 <10 nM.
Sterility
sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-511 is suitable for use as a substrate for culture of various cell types, including ES/iPS cells.
Concentration
500 µg/mL
Source
CHO-S cells expression
Manufacturer
Matrixome Corporation (Japan)


References

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).

Product Specifications

Product Name
iMatrix-511 Stem Cell Culture Substrate
Catalog Number
NP892-01
Size
2x175 µg and 6x175 µg available
Molecular Weight
150 kDa
Purity
>95% pure
Formulation
Purified Laminin-511 E8 proteolytic fragment
Storage and Stability
Store at 4 °C and protect from light exposure. Stable for 2 years from manufacturing date.
Quality Control
Activity: Kd for integrin α6β1 <10 nM.
Sterility
sterile
Notice To Purchaser
REPROCELL is a licensed distributor of Matrixome cell culture substrates to the global market
Recommended Usage
iMatrix-511 is suitable for use as a substrate for culture of various cell types, including ES/iPS cells.
Concentration
500 µg/mL
Source
CHO-S cells expression
Manufacturer
Matrixome Corporation (Japan)

References

  1. Takayama K. et al., "Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells". Biochemical and Biophysical Research Commun. 474 (1): 91-96 (2016).
  2. Nishimura K. et al., "Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1".  Stem Cell Reports 6 (4): 511-524 (2016).
  3. Matsuno K. et al., "Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stemcells". Differentiation 2016.04.002.
  4. Hayashi R. et al., "Co-ordinated ocular development ofrom human iPS cells and recovery of corneal function". Nature 531, 368-80 (2016),
  5. Sasaki K. et al., "Robust in vitro induction of human germ celll fate from pluripotent stem cells". Cell Stem Cell 17 (2):178-194 (2015).
  6. Okumura N. et al., "Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells". Invest Ophthalmal Vis Sci. 56 (5), 2933-42 (2015).
  7. Nakagawa M. et al., "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells:. Scientific Reports 4: 3594 (2014).
  8. Miyazaki T. et al. "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotentn stem cells.  Nature Communications 3: 1236 (2012).
  9. Taniguchi Y. et al., "The C-terminal region of laminin beta-chains modulates the integrin-binding affinities of laminins:" J. Biol. Chem. 284 (12): 7820-31 (2009).
  10. Ido H. et al., "The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by lamiinins.  J. Biol. Chem. 282 (15): 11144-54 (2017).