microRNA Booster Kit

Catalog 00-0073
Size 1 kit
Price
$695.00
$695.00
Availability In stock
Catalog
00-0073

The Stemgent microRNA Booster Kit enhances, and simplifies mRNA reprogramming for fast, efficient iPS cell line generation from both normal and patient specific cell lines.The microRNA Booster Kit includes the microRNA Reprogramming Cocktail and B18R Recombinant Protein.

The microRNA-enhanced mRNA Reprogramming method improves upon the first generation mRNA Reprogramming Kit, delivering faster reprogramming kinetics, a streamlined protocol and improved efficacy on hard-to-reprogram or refractory cell types. The microRNA-enhanced method combines Stemgent’s mRNA Reprogramming Kit with the microRNA Booster Kit and the Stemfect™ RNA Transfection Kit to generate expandable primary iPS cell colonies in as little as 8 days. This new method improves kinetics and reduces mRNA dose per transfection resulting in a per well cost reduction of approximately 35% to 45% versus feeder-based mRNA Reprogramming.

Advantages

Stemgent
mRNA Reprogramming Method
Stemgent
Enhanced microRNA
mRNA Reprogramming Method
Reprogramming efficiency >1% >1%
Fast Reprogramming Kinetics Yes Yes
Somatic Cell → iPS Cell 3 weeks 2 weeks
Risk of insertional mutagenesis None None
Screening for Viral Clearance None None
Screening for DNA vector retention and/or integration None None
Bio-containment issues associated with viral applications None None
# of wells per kit
(6 well plate format)
4 9-10
(35%-45% per well cost reduction)
Reprogramming Substrate Fibroblast feeder layer Extracellular Matrix
Captures hard-to reprogram and refractory cell types Yes
Simple, user friendly protocol (overnight transfections) Yes



Product Specifications

Product Name
microRNA Booster Kit
Catalog Number
00-0073
Size
1 kit
Storage and Stability
Store both the microRNA Reprogramming Cocktail and the B18R protein at or below -70 °C.
Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
Quality Control
The Stemgent microRNA Booster Kit is functionally validated for successful RNA-based reprogramming of human newborn foreskin fibroblasts. All components of the kit are sterile and have tested negative for mycoplasma.
Related Products
RNA Transfection Kit (00-0069)
mRNA Reprogramming Kit (00-0071)
NuFF-R IRR (GSC3002G)
hPSC XF Medium for Human iPS and ES Cells (01-0005)
FGF-basic, Human Recombinant (03-0002)
Stemgent StainAlive TRA-1-60 Antibody (DyLight 488), Mouse anti-Human (09-0068)
Stemgent StainAlive TRA-1-81 Antibody (DyLight 488), Mouse anti-Human (09-0069)
Stemgent Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human (09-0020)
Stemgent Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human (09-0023)
Stemgent SSEA-4 Antibody (Purified), Mouse anti-Human (09-0006)
Stemgent TRA-1-60 Antibody (Purified), Mouse anti-Human (09-0010)
Stemgent TRA-1-81 Antibody (Purified), Mouse anti-Human (09-0011)
mRNA Reprogramming Factors Set: hOKSML (00-0067)
Recommended Usage
Refer to the microRNA-Enhanced mRNA Reprogramming Protocol for directions on use of the Stemgent microRNA Booster Kit. Refer to the protocol Generating NuFF-Conditioned Pluriton Medium for instructions on production of the required reprogramming medium. Refer to the User Manual: Stemgent mRNA Reprogramming System for additional details on materials and handling.
Kit Contents
2 vials - microRNA Reprogramming Cocktail (20 µM) (Cat. No. 05-0036), 35 µL
B18R Recombinant Protein, Carrier-Free (Cat. No. 03-0017), 50 µg


Product Specifications

Product Name
microRNA Booster Kit
Catalog Number
00-0073
Size
1 kit
Storage and Stability
Store both the microRNA Reprogramming Cocktail and the B18R protein at or below -70 °C.
Kit components are stable for a minimum of 3 months from date of receipt when stored as directed.
Quality Control
The Stemgent microRNA Booster Kit is functionally validated for successful RNA-based reprogramming of human newborn foreskin fibroblasts. All components of the kit are sterile and have tested negative for mycoplasma.
Related Products
RNA Transfection Kit (00-0069)
mRNA Reprogramming Kit (00-0071)
NuFF-R IRR (GSC3002G)
hPSC XF Medium for Human iPS and ES Cells (01-0005)
FGF-basic, Human Recombinant (03-0002)
Stemgent StainAlive TRA-1-60 Antibody (DyLight 488), Mouse anti-Human (09-0068)
Stemgent StainAlive TRA-1-81 Antibody (DyLight 488), Mouse anti-Human (09-0069)
Stemgent Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human (09-0020)
Stemgent Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human (09-0023)
Stemgent SSEA-4 Antibody (Purified), Mouse anti-Human (09-0006)
Stemgent TRA-1-60 Antibody (Purified), Mouse anti-Human (09-0010)
Stemgent TRA-1-81 Antibody (Purified), Mouse anti-Human (09-0011)
mRNA Reprogramming Factors Set: hOKSML (00-0067)
Recommended Usage
Refer to the microRNA-Enhanced mRNA Reprogramming Protocol for directions on use of the Stemgent microRNA Booster Kit. Refer to the protocol Generating NuFF-Conditioned Pluriton Medium for instructions on production of the required reprogramming medium. Refer to the User Manual: Stemgent mRNA Reprogramming System for additional details on materials and handling.
Kit Contents
2 vials - microRNA Reprogramming Cocktail (20 µM) (Cat. No. 05-0036), 35 µL
B18R Recombinant Protein, Carrier-Free (Cat. No. 03-0017), 50 µg