IPS-Spheres

Catalog BR-01S-50
BR-01S-150
Size 50 mg (Cat. No. BR-01S-50)
150 mg (Cat. No. BR-01S-150)
Price
$0.00
$0.00
Availability Out of stock
Catalog
BR-01S

IPS-SpheresTM microcarriers (MCs) provide a foundation for the scalable and robust production of human pluripotent stem cell (hPSCs)-derived functional cells in large numbers, by means of an integrated propagation and differentiation bioprocess in a defined environment [1]. The MCs can be used for generating uniform EBs-like cell aggregates under agitation cultures and subsequently directly differentiated towards various lineages or functional cells (e.g. cardiomyocytes [2,3,4] and neural progenitor cells [5]). The microcarrier beads are pre-coated with human laminin protein.  The advantages of this approach are: high cell yields; scalable; controlled aggregate size; negligible labor-intensive manual intervention allowing expansion and differentiation of hPSCs in one vessel unit [1,2].

Applications include:

  • Enables high cell yields for hPSCs expansion
  • Can be used for directed differentiation to any lineages or functional cells with different medium, such as cardiomyocytes and neuroprogenitors
  • Alternative to EBs formation, giving higher cell yields and easier handling than “cutting colonies” or Aggrewells


Product Specifications

Product Name
IPS-Spheres
Catalog Number
BR-01S
Size
50 mg (Cat. No. BR-01S-50)
150 mg (Cat. No. BR-01S-150)
Storage and Stability
Store at 4℃, stable for 1 year
Sterility
Sterile
Recommended Usage
For videos and protocols on use of IPS-Spheres, visit the website of Brilliant Research to find the most up-to-date information (https://brilliant-research.com)
Manufacturer
Brilliant Research Pte., Ltd. (Singapore)

  1. Sivalingam J et al. 'Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform'. Tissue Eng Part C Methods. 2016 Aug;22(8):765-80.
  2. Lam AT et al. ‘Integrated processes for expansion and differentiation of human pluripotent stem cells in suspended microcarriers cultures’ Biochem Biophys Res Commun. 2015 Sep 15.
  3. Lam AT et al. ‘Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture’. Stem Cell Res Ther. 2014 Sep 15;5(5):110.
  4. Lam AT et al. ‘Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures’. Biores Open Access. 2015 Apr 1;4(1):242-57.
  5. Ting S et al. ‘An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures’. Stem Cell Res. 2014 Sep;13(2):202-13.
  6. Brady J et al. ‘Microcarrier suspension cultures for high-density expansion and differentiation of human pluripotent stem cells to neural progenitor cells’. Tissue Eng Part C Methods. 2013 Feb;19(2):166-80.

Product Specifications

Product Name
IPS-Spheres
Catalog Number
BR-01S
Size
50 mg (Cat. No. BR-01S-50)
150 mg (Cat. No. BR-01S-150)
Storage and Stability
Store at 4℃, stable for 1 year
Sterility
Sterile
Recommended Usage
For videos and protocols on use of IPS-Spheres, visit the website of Brilliant Research to find the most up-to-date information (https://brilliant-research.com)
Manufacturer
Brilliant Research Pte., Ltd. (Singapore)

  1. Sivalingam J et al. 'Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platform'. Tissue Eng Part C Methods. 2016 Aug;22(8):765-80.
  2. Lam AT et al. ‘Integrated processes for expansion and differentiation of human pluripotent stem cells in suspended microcarriers cultures’ Biochem Biophys Res Commun. 2015 Sep 15.
  3. Lam AT et al. ‘Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture’. Stem Cell Res Ther. 2014 Sep 15;5(5):110.
  4. Lam AT et al. ‘Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures’. Biores Open Access. 2015 Apr 1;4(1):242-57.
  5. Ting S et al. ‘An intermittent rocking platform for integrated expansion and differentiation of human pluripotent stem cells to cardiomyocytes in suspended microcarrier cultures’. Stem Cell Res. 2014 Sep;13(2):202-13.
  6. Brady J et al. ‘Microcarrier suspension cultures for high-density expansion and differentiation of human pluripotent stem cells to neural progenitor cells’. Tissue Eng Part C Methods. 2013 Feb;19(2):166-80.